Construct: ORF TRCN0000468005
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004019.1_s317c1
- Derived from:
- ccsbBroadEn_14872
- DNA Barcode:
- TCGGGCCTGTCTTATCTTATATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBB (7314)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468005
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321022.2 | 54.1% | 36.7% | (many diffs) |
| 2 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001033930.3 | 50.2% | 59.3% | (many diffs) |
| 3 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321017.1 | 50.2% | 59.3% | (many diffs) |
| 4 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321018.2 | 50.2% | 59.3% | (many diffs) |
| 5 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321019.2 | 50.2% | 59.3% | (many diffs) |
| 6 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321020.2 | 50.2% | 59.3% | (many diffs) |
| 7 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_003333.5 | 50.2% | 59.3% | (many diffs) |
| 8 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | NM_001321021.1 | 47.6% | 56.2% | (many diffs) |
| 9 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_005260052.2 | 41.2% | 48.7% | (many diffs) |
| 10 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_005260053.3 | 41.2% | 48.7% | (many diffs) |
| 11 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_005260054.2 | 41.2% | 48.7% | (many diffs) |
| 12 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_006722871.2 | 41.2% | 48.7% | (many diffs) |
| 13 | human | 6233 | RPS27A | ribosomal protein S27a | NM_001135592.2 | 40.5% | 48.7% | (many diffs) |
| 14 | human | 6233 | RPS27A | ribosomal protein S27a | NM_001177413.1 | 40.5% | 48.7% | (many diffs) |
| 15 | human | 6233 | RPS27A | ribosomal protein S27a | NM_002954.5 | 40.5% | 48.7% | (many diffs) |
| 16 | human | 7314 | UBB | ubiquitin B | NM_001281716.1 | 32.8% | 33.6% | (many diffs) |
| 17 | human | 7314 | UBB | ubiquitin B | NM_001281717.1 | 32.8% | 33.6% | (many diffs) |
| 18 | human | 7314 | UBB | ubiquitin B | NM_001281718.1 | 32.8% | 33.6% | (many diffs) |
| 19 | human | 7314 | UBB | ubiquitin B | NM_001281719.1 | 32.8% | 33.6% | (many diffs) |
| 20 | human | 7314 | UBB | ubiquitin B | NM_001281720.1 | 32.8% | 33.6% | (many diffs) |
| 21 | human | 7314 | UBB | ubiquitin B | NM_018955.4 | 32.8% | 33.6% | (many diffs) |
| 22 | human | 7311 | UBA52 | ubiquitin A-52 residue ribo... | XM_017027198.1 | 31.6% | 37.4% | (many diffs) |
| 23 | human | 23666 | UBBP4 | ubiquitin B pseudogene 4 | NR_144546.1 | 14.6% | (many diffs) | |
| 24 | human | 7316 | UBC | ubiquitin C | NM_021009.7 | 9.7% | 11% | (many diffs) |
| 25 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348227.1 | 52% | 59.3% | (many diffs) |
| 26 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348228.1 | 52% | 59.3% | (many diffs) |
| 27 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_001348229.1 | 52% | 59.3% | (many diffs) |
| 28 | mouse | 22186 | Uba52 | ubiquitin A-52 residue ribo... | NM_019883.4 | 52% | 59.3% | (many diffs) |
| 29 | mouse | 78294 | Rps27a | ribosomal protein S27A | NM_001033865.1 | 40.9% | 48.7% | (many diffs) |
| 30 | mouse | 78294 | Rps27a | ribosomal protein S27A | NM_024277.2 | 40.9% | 48.7% | (many diffs) |
| 31 | mouse | 218963 | Gm1821 | predicted gene 1821; ubiqui... | NR_002875.2 | 30.1% | (many diffs) | |
| 32 | mouse | 22187 | Ubb | ubiquitin B | NM_001313984.1 | 23% | 24.9% | (many diffs) |
| 33 | mouse | 22187 | Ubb | ubiquitin B | NM_011664.4 | 23% | 24.9% | (many diffs) |
| 34 | mouse | 22190 | Ubc | ubiquitin C | NM_019639.4 | 9.3% | 10.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 212
- ORF end:
- 443
- ORF length:
- 231
- Sequence:
-
1 TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC 61 TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG 121 CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA 181 TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGCAGATC TTCGTGAAAA CCCTTACCGG 241 CAAGACCATC ACCCTTGAGG TGGAGCCCAG TGACACCATC GAAAATGTGA AGGCCAAGAT 301 CCAGGATAAG GAAGGCATTC CCCCCGACCA GCAGAGGCTC ATCTTTGCAG GCAAGCAGCT 361 GGAAGACGGC CGTACTCTTT CTGACTACAA CATCCAGAAG GAGTCGACCC TGCACCTGGT 421 CCTGCGCCTG AGGGGTGGCT GTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 481 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 541 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC GGGCCTGTCT 601 TATCTTATAT CCACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 661 tt