Transcript: Human NR_146128.1

Homo sapiens F-box and leucine rich repeat protein 2 (FBXL2), transcript variant 19, non-coding RNA.

Source:
NCBI, updated 2018-05-10
Taxon:
Homo sapiens (human)
Gene:
FBXL2 (25827)
Length:
1693
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_146128.1
NBCI Gene record:
FBXL2 (25827)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_146128.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000279844 GCTCGGAATTGCCACGAATTG pLKO_005 716 3UTR 100% 10.800 15.120 N FBXL2 n/a
2 TRCN0000004278 CTCTCCATTCACTGTCCTAAA pLKO.1 791 3UTR 100% 10.800 7.560 N FBXL2 n/a
3 TRCN0000279842 CTCTCCATTCACTGTCCTAAA pLKO_005 791 3UTR 100% 10.800 7.560 N FBXL2 n/a
4 TRCN0000004277 GATAACCGACAGCACACTCAT pLKO.1 766 3UTR 100% 4.950 3.465 N FBXL2 n/a
5 TRCN0000092671 CCTTAGCAGATTCTGTTCCAA pLKO.1 244 3UTR 100% 3.000 2.100 N Fbxl2 n/a
6 TRCN0000004276 GAACCTCAATGGATGCACAAA pLKO.1 199 3UTR 100% 4.950 2.970 N FBXL2 n/a
7 TRCN0000279774 GAACCTCAATGGATGCACAAA pLKO_005 199 3UTR 100% 4.950 2.970 N FBXL2 n/a
8 TRCN0000004280 GTCTATTACAAACAGCTCCTT pLKO.1 295 3UTR 100% 2.640 1.584 N FBXL2 n/a
9 TRCN0000437561 ATCACTGACAGCACGTGTTAC pLKO_005 221 3UTR 100% 10.800 7.560 N Fbxl2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_146128.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02865 pDONR223 100% 54.4% None 1_97del;162_163ins225;1142_1693del n/a
2 ccsbBroad304_02865 pLX_304 0% 54.4% V5 1_97del;162_163ins225;1142_1693del n/a
3 TRCN0000480745 CATTCGACCGAATATATATCTGAC pLX_317 31.4% 54.4% V5 1_97del;162_163ins225;1142_1693del n/a
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