Construct: ORF TRCN0000480745

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000026.2_s317c1
Derived from:
ccsbBroadEn_02865
DNA Barcode:
CATTCGACCGAATATATATCTGAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FBXL2 (25827)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480745
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25827 FBXL2 F-box and leucine rich repe... NM_012157.5 100% 100%
2 human 25827 FBXL2 F-box and leucine rich repe... NM_001349316.2 93.1% 93.1% 951_1043del
3 human 25827 FBXL2 F-box and leucine rich repe... XM_024453440.1 93.1% 93.1% 951_1043del
4 human 25827 FBXL2 F-box and leucine rich repe... NM_001349322.2 78.3% 77.5% (many diffs)
5 human 25827 FBXL2 F-box and leucine rich repe... NM_001349323.1 72.5% 71.2% 0_1ins334;23_41del
6 human 25827 FBXL2 F-box and leucine rich repe... NM_001349324.2 72.5% 71.2% 0_1ins334;23_41del
7 human 25827 FBXL2 F-box and leucine rich repe... NM_001349325.2 72.5% 71.2% 0_1ins334;23_41del
8 human 25827 FBXL2 F-box and leucine rich repe... NM_001349326.2 72.5% 71.2% 0_1ins334;23_41del
9 human 25827 FBXL2 F-box and leucine rich repe... NM_001349319.1 67.7% 66.4% 0_1ins334;23_41del;636_728del
10 human 25827 FBXL2 F-box and leucine rich repe... NM_001349320.2 67.7% 66.4% 0_1ins334;23_41del;636_728del
11 human 25827 FBXL2 F-box and leucine rich repe... NM_001349321.2 67.7% 66.4% 0_1ins334;23_41del;636_728del
12 human 25827 FBXL2 F-box and leucine rich repe... XM_005265015.3 67.7% 66.4% 0_1ins334;23_41del;636_728del
13 human 25827 FBXL2 F-box and leucine rich repe... XM_011533559.2 67.7% 66.4% 0_1ins334;23_41del;636_728del
14 human 25827 FBXL2 F-box and leucine rich repe... XM_017006093.1 67.7% 66.4% 0_1ins334;23_41del;636_728del
15 human 25827 FBXL2 F-box and leucine rich repe... NR_146127.1 66.1% 1_97del;1367_1918del
16 human 25827 FBXL2 F-box and leucine rich repe... NR_146125.1 63% 1_97del;162_255del;1461_2012del
17 human 25827 FBXL2 F-box and leucine rich repe... NR_146124.1 62.5% (many diffs)
18 human 25827 FBXL2 F-box and leucine rich repe... NR_146129.1 60.2% (many diffs)
19 human 25827 FBXL2 F-box and leucine rich repe... NR_146128.1 54.4% 1_97del;162_163ins225;1142_1693del
20 human 25827 FBXL2 F-box and leucine rich repe... NR_146132.2 52% 1_71del;1341_2438del
21 human 25827 FBXL2 F-box and leucine rich repe... NR_146123.2 50.1% 1_71del;136_229del;1435_2532del
22 human 25827 FBXL2 F-box and leucine rich repe... NR_146121.1 42.2% 1_71delinsAT;1339_2998del
23 human 25827 FBXL2 F-box and leucine rich repe... NR_146122.1 40.9% 1_71delinsAT;134_227del;1433_3092del
24 human 25827 FBXL2 F-box and leucine rich repe... NR_146133.2 32.7% 1_71del;137_186del;1391_3877del
25 human 25827 FBXL2 F-box and leucine rich repe... NR_146126.2 32.4% 1_71del;137_214del;1419_3905del
26 human 25827 FBXL2 F-box and leucine rich repe... NR_146135.2 32.4% 1_71del;135_221del;1428_3914del
27 human 25827 FBXL2 F-box and leucine rich repe... NR_146131.2 32.3% 1_71del;136_229del;1435_3921del
28 human 25827 FBXL2 F-box and leucine rich repe... NR_146134.2 31.6% (many diffs)
29 human 25827 FBXL2 F-box and leucine rich repe... NR_146130.2 31% (many diffs)
30 mouse 72179 Fbxl2 F-box and leucine-rich repe... NM_178624.6 87.9% 95.9% (many diffs)
31 mouse 72179 Fbxl2 F-box and leucine-rich repe... XR_379880.3 71% (many diffs)
32 mouse 72179 Fbxl2 F-box and leucine-rich repe... XR_379879.2 65.2% (many diffs)
33 mouse 72179 Fbxl2 F-box and leucine-rich repe... XR_379878.3 64.1% (many diffs)
34 mouse 72179 Fbxl2 F-box and leucine-rich repe... XM_011243027.1 63.8% 69% (many diffs)
35 mouse 72179 Fbxl2 F-box and leucine-rich repe... XM_011243028.2 63.8% 69% (many diffs)
36 mouse 72179 Fbxl2 F-box and leucine-rich repe... XM_006512295.2 63.4% 68.5% (many diffs)
37 mouse 72179 Fbxl2 F-box and leucine-rich repe... XM_006512294.3 63.1% 69% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1335
ORF length:
1269
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt tttctcaaac aatgatgaag gccttattaa caaaaagtta cccaaagaac
121ttctgttaag aatattttcc ttcttggata tagtaacttt gtgccgatgt gcacagattt
181ccaaggcttg gaacatctta gccctggatg gaagcaactg gcaaagaata gatcttttta
241actttcaaac agatgtagag ggtcgagtgg tggaaaatat ctcgaagcga tgcggtggat
301tcctgaggaa gctcagcttg cgaggctgca ttggtgttgg ggattcctcc ttgaagacct
361ttgcacagaa ctgccgaaac attgaacatt tgaacctcaa tggatgcaca aaaatcactg
421acagcacgtg ttatagcctt agcagattct gttccaagct gaaacatctg gatctgacct
481cctgtgtgtc tattacaaac agctccttga aggggatcag tgagggctgc cgaaacctgg
541agtacctgaa cctctcttgg tgtgatcaga tcacgaagga tggcatcgag gcactggtgc
601gaggttgtcg aggcctgaaa gccctgctcc tgaggggctg cacacagtta gaagatgaag
661ctctgaaaca cattcagaat tactgccatg agcttgtgag cctcaacttg cagtcctgct
721cacgtatcac ggatgaaggt gtggtgcaga tatgcagggg ctgtcaccgg ctacaggctc
781tctgcctttc gggttgcagc aacctcacag atgcctctct tacagccctg ggtttgaact
841gtccgcgact gcaaattttg gaggctgccc gatgctccca tttgactgac gcaggtttta
901cacttttagc tcggaattgc cacgaattgg agaagatgga TCTTGAAGAA TGCATCCTGA
961TAACCGACAG CACACTCATC CAGCTCTCCA TTCACTGTCC TAAACTGCAA GCCCTGAGCC
1021TGTCCCACTG TGAACTCATC ACAGATGATG GGATCCTGCA CCTGAGCAAC AGTACCTGTG
1081GCCATGAGAG GCTGCGGGTA CTGGAGTTGG ACAACTGCCT CCTCATCACT GATGTGGCCC
1141TGGAACACCT AGAGAACTGC CGAGGCCTGG AGCGCCTCGA GCTGTACGAC TGCCAGCAGG
1201TTACCCGTGC AGGCATCAAG CGGATGCGGG CTCAGCTCCC TCATGTCAAA GTCCACGCCT
1261ACTTTGCTCC CGTCACCCCA CCGACAGCAG TGGCAGGAAG TGGACAGCGA CTGTGCAGGT
1321GCTGTGTCAT TCTCTGCCCA ACTATCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1381CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1441TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CATTCGACCG AATATATATC
1501TGACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)