Transcript: Human NR_148893.2

Homo sapiens phospholipid phosphatase 5 (PLPP5), transcript variant 10, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
PLPP5 (84513)
Length:
2261
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_148893.2
NBCI Gene record:
PLPP5 (84513)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_148893.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000295998 CTACGTGGAGGCGGAGTATTT pLKO_005 169 3UTR 100% 13.200 18.480 N PLPP5 n/a
2 TRCN0000040241 AGCCGATGTTTGTTATTGCAT pLKO.1 198 3UTR 100% 3.000 4.200 N PLPP5 n/a
3 TRCN0000040238 CGTCTTTACCAACACAATAAA pLKO.1 328 3UTR 100% 15.000 12.000 N PLPP5 n/a
4 TRCN0000298906 CGTCTTTACCAACACAATAAA pLKO_005 328 3UTR 100% 15.000 12.000 N PLPP5 n/a
5 TRCN0000296043 TGATCTTCCTGGCCAAATTTC pLKO_005 237 3UTR 100% 13.200 9.240 N PLPP5 n/a
6 TRCN0000040239 CCTTTCTGTCACCTCTACTTT pLKO.1 712 3UTR 100% 5.625 3.938 N PLPP5 n/a
7 TRCN0000040242 GACACAAGAGACAGCAGACAA pLKO.1 269 3UTR 100% 4.950 3.465 N PLPP5 n/a
8 TRCN0000298907 GACACAAGAGACAGCAGACAA pLKO_005 269 3UTR 100% 4.950 3.465 N PLPP5 n/a
9 TRCN0000040240 CCCAGTGGACATTCTTCCTTT pLKO.1 597 3UTR 100% 4.950 2.970 N PLPP5 n/a
10 TRCN0000080722 TCTGTGCCTTTCTGTCACCTT pLKO.1 706 3UTR 100% 2.640 1.584 N Plpp5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_148893.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12825 pDONR223 100% 29.5% None 1_148del;364_490del;945_2261del n/a
2 ccsbBroad304_12825 pLX_304 0% 29.5% V5 1_148del;364_490del;945_2261del n/a
3 TRCN0000467220 ATTTTGTGCCGACTTTTCAACGTC pLX_317 59.3% 29.5% V5 1_148del;364_490del;945_2261del n/a
4 ccsbBroadEn_16036 pDONR223 0% 23% None (many diffs) n/a
5 ccsbBroad304_16036 pLX_304 0% 23% V5 (many diffs) n/a
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