Transcript: Human NR_157087.1

Homo sapiens adaptor related protein complex 3 subunit sigma 1 (AP3S1), transcript variant 13, non-coding RNA.

Source:
NCBI, updated 2018-06-07
Taxon:
Homo sapiens (human)
Gene:
AP3S1 (1176)
Length:
1193
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_157087.1
NBCI Gene record:
AP3S1 (1176)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_157087.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000060315 CCATGTAGACAAGGTTCACAA pLKO.1 354 3UTR 100% 4.950 3.960 N AP3S1 n/a
2 TRCN0000290102 CCATGTAGACAAGGTTCACAA pLKO_005 354 3UTR 100% 4.950 3.960 N AP3S1 n/a
3 TRCN0000379473 CATTGGTGACATCAGTATAAA pLKO_005 555 3UTR 100% 15.000 10.500 N AP3S1 n/a
4 TRCN0000296527 CAAGTATTTGTGGAAACATTA pLKO_005 292 3UTR 100% 13.200 9.240 N AP3S1 n/a
5 TRCN0000113166 CCGTGCTGTATCAGCTGTAAA pLKO.1 498 3UTR 100% 13.200 9.240 N Ap3s1 n/a
6 TRCN0000060316 CTTCCTGAGATCCCAAGAAAT pLKO.1 529 3UTR 100% 13.200 9.240 N AP3S1 n/a
7 TRCN0000290032 CTTCCTGAGATCCCAAGAAAT pLKO_005 529 3UTR 100% 13.200 9.240 N AP3S1 n/a
8 TRCN0000380624 ACTTTCCATTTGGTATCTAAG pLKO_005 124 3UTR 100% 10.800 7.560 N AP3S1 n/a
9 TRCN0000381405 CACAAGTGCAGCATTCCTTAA pLKO_005 960 3UTR 100% 10.800 7.560 N AP3S1 n/a
10 TRCN0000113165 CCAAGTATGTATTGCATGAAA pLKO.1 1020 3UTR 100% 5.625 3.938 N Ap3s1 n/a
11 TRCN0000309509 CCAAGTATGTATTGCATGAAA pLKO_005 1020 3UTR 100% 5.625 3.938 N Ap3s1 n/a
12 TRCN0000296529 ACTGATTTATAGACATTATGC pLKO_005 207 3UTR 100% 4.950 3.465 N AP3S1 n/a
13 TRCN0000296526 GATCTAATTCAAGTATTTGTG pLKO_005 283 3UTR 100% 4.950 3.465 N AP3S1 n/a
14 TRCN0000060314 GCAAATCATCAGGGAGACTTT pLKO.1 108 3UTR 100% 4.950 3.465 N AP3S1 n/a
15 TRCN0000060317 GTAATTTCCTAGAAGGAGGAT pLKO.1 161 3UTR 100% 2.640 1.848 N AP3S1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_157087.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00320 pDONR223 100% 44.8% None (many diffs) n/a
2 ccsbBroad304_00320 pLX_304 0% 44.8% V5 (many diffs) n/a
3 TRCN0000474919 TGCGCCCAAACAGCAAGTCATGCG pLX_317 69.4% 44.8% V5 (many diffs) n/a
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