Construct: ORF TRCN0000474919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002811.1_s317c1
- Derived from:
- ccsbBroadEn_00320
- DNA Barcode:
- TGCGCCCAAACAGCAAGTCATGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP3S1 (1176)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474919
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001284.4 | 100% | 100% | |
| 2 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001364119.1 | 92.3% | 92.3% | 452_499del |
| 3 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001002924.3 | 88.1% | 88% | 0_1ins67;2_3delTGinsC |
| 4 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001318091.2 | 87.5% | 87.5% | 273_274ins72 |
| 5 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001318093.2 | 82.3% | 78.7% | (many diffs) |
| 6 | human | 1176 | AP3S1 | adaptor related protein com... | XM_011543136.3 | 82.3% | 78.7% | (many diffs) |
| 7 | human | 1176 | AP3S1 | adaptor related protein com... | XM_017009024.2 | 82.3% | 78.7% | (many diffs) |
| 8 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001318094.2 | 81.3% | 81.3% | 343_344ins108 |
| 9 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001364120.1 | 69.9% | 66.3% | (many diffs) |
| 10 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001364122.1 | 68.9% | 68.9% | 273_274ins180 |
| 11 | human | 1176 | AP3S1 | adaptor related protein com... | NM_001318090.2 | 64.7% | 64.7% | 69_70ins204 |
| 12 | human | 1176 | AP3S1 | adaptor related protein com... | XM_017009025.1 | 46% | 43.6% | (many diffs) |
| 13 | human | 1176 | AP3S1 | adaptor related protein com... | NR_157087.1 | 44.8% | (many diffs) | |
| 14 | human | 1176 | AP3S1 | adaptor related protein com... | NR_157084.1 | 42.7% | 1_92del;544_632del;761_1353del | |
| 15 | human | 1176 | AP3S1 | adaptor related protein com... | NR_157085.1 | 41.2% | 1_92del;435_575del;813_1405del | |
| 16 | human | 1176 | AP3S1 | adaptor related protein com... | NR_157086.1 | 32.8% | (many diffs) | |
| 17 | mouse | 11777 | Ap3s1 | adaptor-related protein com... | NM_009681.5 | 95.1% | 100% | (many diffs) |
| 18 | mouse | 11777 | Ap3s1 | adaptor-related protein com... | NM_001303015.1 | 77.5% | 78.7% | (many diffs) |
| 19 | mouse | 11777 | Ap3s1 | adaptor-related protein com... | XM_006525526.2 | 77.5% | 78.7% | (many diffs) |
| 20 | mouse | 11777 | Ap3s1 | adaptor-related protein com... | XM_006525528.3 | 62.5% | 64.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 645
- ORF length:
- 579
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat caaggcgatc ctaatcttca acaaccacgg gaagccgcgg ctctccaagt 121 tctaccagcc ctacagtgaa gatacacaac agcaaatcat cagggagact ttccatttgg 181 tatctaagag agatgaaaat gtttgtaatt tcctagaagg aggattatta attggaggaT 241 CTGACAACAA ACTGATTTAT AGACATTATG CAACGTTATA TTTTGTCTTC TGTGTGGATT 301 CTTCAGAAAG TGAACTTGGC ATTTTAGATC TAATTCAAGT ATTTGTGGAA ACATTAGACA 361 AATGTTTTGA AAATGTCTGT GAGCTGGATT TGATTTTCCA TGTAGACAAG GTTCACAATA 421 TTCTTGCAGA AATGGTGATG GGGGGAATGG TATTGGAGAC AAATATGAAT GAGATTGTTA 481 CACAAATTGA TGCACAAAAT AAGCTGGAAA AATCTGAGGC TGGCTTAGCA GGAGCTCCAG 541 CCCGTGCTGT ATCAGCTGTA AAGAATATGA ATCTTCCTGA GATCCCAAGA AATATTAACA 601 TTGGTGACAT CAGTATAAAA GTGCCAAACC TGCCCTCTTT TAAATACCCA ACTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 781 GAAAGGACGA TGCGCCCAAA CAGCAAGTCA TGCGACGCGT TAAGTCgaca atcaacctct 841 ggattacaaa atttgtgaaa gatt