Transcript: Human NR_157126.1

Homo sapiens DEP domain containing 5, GATOR1 subcomplex subunit (DEPDC5), transcript variant 12, non-coding RNA.

Source:
NCBI, updated 2019-08-31
Taxon:
Homo sapiens (human)
Gene:
DEPDC5 (9681)
Length:
5099
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_157126.1
NBCI Gene record:
DEPDC5 (9681)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_157126.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000137028 CCAAATCATAGTGCAGCCCAA pLKO.1 2522 3UTR 100% 2.160 3.024 N DEPDC5 n/a
2 TRCN0000134778 CCATTGTTCAAGCTCCATAAT pLKO.1 1221 3UTR 100% 13.200 10.560 N DEPDC5 n/a
3 TRCN0000135857 CCCTTGACCTAGTGGAATTAA pLKO.1 376 3UTR 100% 15.000 10.500 N DEPDC5 n/a
4 TRCN0000136459 CCTTCACCATCTATGGGATAT pLKO.1 4941 3UTR 100% 10.800 7.560 N DEPDC5 n/a
5 TRCN0000135505 GCTTCTTGTTAGTCCCAGTTT pLKO.1 4007 3UTR 100% 4.950 3.465 N DEPDC5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_157126.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02226 pDONR223 100% 32.8% None (many diffs) n/a
2 ccsbBroad304_02226 pLX_304 0% 32.8% V5 (many diffs) n/a
3 TRCN0000471584 ACGCCCCGTCACTGCACAAAGTGG pLX_317 23.1% 32.8% V5 (many diffs) n/a
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