Transcript: Human NR_158781.1

Homo sapiens zinc finger and BTB domain containing 8 opposite strand (ZBTB8OS), transcript variant 33, non-coding RNA.

Source:
NCBI, updated 2019-03-22
Taxon:
Homo sapiens (human)
Gene:
ZBTB8OS (339487)
Length:
1802
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_158781.1
NBCI Gene record:
ZBTB8OS (339487)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_158781.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000131054 CCAAGTATCCGCCAGTCAATA pLKO.1 468 3UTR 100% 13.200 9.240 N ZBTB8OS n/a
2 TRCN0000312431 CCAAGTATCCGCCAGTCAATA pLKO_005 468 3UTR 100% 13.200 9.240 N ZBTB8OS n/a
3 TRCN0000130501 GCAATGCAGGTCTATAATGAA pLKO.1 704 3UTR 100% 5.625 3.938 N ZBTB8OS n/a
4 TRCN0000312490 GCAATGCAGGTCTATAATGAA pLKO_005 704 3UTR 100% 5.625 3.938 N ZBTB8OS n/a
5 TRCN0000129046 GACTTACAGTCTCTTCTGTTT pLKO.1 506 3UTR 100% 4.950 3.465 N ZBTB8OS n/a
6 TRCN0000341019 CCGGGAAGTGAAAGTACTTAA pLKO_005 580 3UTR 100% 13.200 18.480 N Zbtb8os n/a
7 TRCN0000215857 CGGGAAGTGAAAGTACTTAAT pLKO.1 581 3UTR 100% 13.200 18.480 N Zbtb8os n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_158781.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13588 pDONR223 100% 13.3% None (many diffs) n/a
2 ccsbBroad304_13588 pLX_304 0% 13.3% V5 (many diffs) n/a
3 TRCN0000469966 TAAGGACCTGGCTTGGCCGCGGTA pLX_317 100% 13.3% V5 (many diffs) n/a
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