Transcript: Human NR_161388.1

Homo sapiens ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 6, non-coding RNA.

Source:
NCBI, updated 2019-04-10
Taxon:
Homo sapiens (human)
Gene:
ARAP1 (116985)
Length:
4628
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_161388.1
NBCI Gene record:
ARAP1 (116985)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_161388.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000047836 CCGCAGGGATCTTACATCTAT pLKO.1 997 3UTR 100% 5.625 7.875 N ARAP1 n/a
2 TRCN0000047837 CGATCGCTACTTCATCCTCAA pLKO.1 3613 3UTR 100% 4.050 5.670 N ARAP1 n/a
3 TRCN0000047833 CCTGCGATACTTTGACAGTAA pLKO.1 1050 3UTR 100% 4.950 3.960 N ARAP1 n/a
4 TRCN0000428992 CATGAGTGGGTCAAGTGTATT pLKO_005 2376 3UTR 100% 13.200 9.240 N ARAP1 n/a
5 TRCN0000433585 ACTTCATCTGCACAGTGTATC pLKO_005 3231 3UTR 100% 10.800 7.560 N ARAP1 n/a
6 TRCN0000418757 CACTTATCGAGCTCTTCTTAC pLKO_005 1660 3UTR 100% 10.800 7.560 N ARAP1 n/a
7 TRCN0000438499 GAGAACGGCTGTACCTGTTTG pLKO_005 2332 3UTR 100% 10.800 7.560 N ARAP1 n/a
8 TRCN0000438169 GCTGGAGAGATGGGCATAAGT pLKO_005 4310 3UTR 100% 5.625 3.938 N ARAP1 n/a
9 TRCN0000047834 CATGGCTTTGAGCACACCTTT pLKO.1 2295 3UTR 100% 4.950 3.465 N ARAP1 n/a
10 TRCN0000047835 GTGGCCTATTAAGAGTCTCAA pLKO.1 3688 3UTR 100% 4.950 3.465 N ARAP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_161388.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09439 pDONR223 100% 63.8% None (many diffs) n/a
2 ccsbBroad304_09439 pLX_304 0% 63.8% V5 (many diffs) n/a
3 TRCN0000480589 AAATAACGGCTCGCGCACATTAAT pLX_317 10.6% 63.8% V5 (many diffs) n/a
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