Construct: ORF TRCN0000480589
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014354.1_s317c1
- Derived from:
- ccsbBroadEn_09439
- DNA Barcode:
- AAATAACGGCTCGCGCACATTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARAP1 (116985)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480589
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116985 | ARAP1 | ArfGAP with RhoGAP domain, ... | NM_001135190.1 | 99.9% | 99.9% | 181C>T;1857G>A |
| 2 | human | 116985 | ARAP1 | ArfGAP with RhoGAP domain, ... | NM_001369489.1 | 94.8% | 94.8% | 181C>T;1073_1255del;2040G>A |
| 3 | human | 116985 | ARAP1 | ArfGAP with RhoGAP domain, ... | NM_015242.4 | 93.9% | 93.9% | (many diffs) |
| 4 | human | 116985 | ARAP1 | ArfGAP with RhoGAP domain, ... | NM_001040118.2 | 78% | 78% | (many diffs) |
| 5 | human | 116985 | ARAP1 | ArfGAP with RhoGAP domain, ... | NR_161388.1 | 63.8% | (many diffs) | |
| 6 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | NM_001040112.1 | 82.3% | 87.5% | (many diffs) |
| 7 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | NM_027180.3 | 82.3% | 87.5% | (many diffs) |
| 8 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508190.1 | 81.5% | 86.7% | (many diffs) |
| 9 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508191.3 | 81.5% | 86.7% | (many diffs) |
| 10 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508192.1 | 81.5% | 86.7% | (many diffs) |
| 11 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508189.2 | 81.2% | 86.2% | (many diffs) |
| 12 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508193.1 | 77.6% | 82.7% | (many diffs) |
| 13 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | NM_001040111.1 | 68.2% | 72.5% | (many diffs) |
| 14 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508186.3 | 67.7% | 71.9% | (many diffs) |
| 15 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508187.1 | 67.7% | 71.9% | (many diffs) |
| 16 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508188.3 | 67.7% | 71.9% | (many diffs) |
| 17 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_011241897.2 | 67.7% | 71.9% | (many diffs) |
| 18 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508196.3 | 63.2% | 65.6% | (many diffs) |
| 19 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | XM_006508195.3 | 62.6% | 65% | (many diffs) |
| 20 | mouse | 69710 | Arap1 | ArfGAP with RhoGAP domain, ... | NM_198096.1 | 56.3% | 60.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3468
- ORF length:
- 3399
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccaagaag gaggagcccc caccgagccg agtcccacgg gccgtgcgcg 121 tggccagtct gctgagcgag ggagaggaac tgtctgggga cgaccaaggg gatgaggaag 181 aggatgacca cgcctatgag ggcgtcccca atggcggatg gcataccagc agcctgagct 241 tgtccttgtc cagcacaata gctgcgccac accccatgga cgggccgcct gggggctcca 301 cccccgtcac accagtcatc aaggctggct ggctggacaa gaacccaccg cagggatctt 361 acatctatca gaaacgatgg gtgagactgg atactgatca cctgcgatac tttgacagta 421 acaaggacgc ttactctaag cgctttatct ctgtggcctg catctcccac gtggctgcca 481 tcggggacca gaagtttgaa gtgatcacaa acaaccgaac ctttgccttc cgggcagaga 541 gtgatgtgga gcggaaggag tggatgcagg ccctgcagca ggccatggct gagcagcgtg 601 cccgggcccg gctctctagc gcttatctgc tgggagttcc aggctcagag cagcctgacc 661 gcgctggcag cctggagctt cgtggcttca agaataagct gtacgtggcc gtggtcgggg 721 acaaagtgca gctctacaag aatctagagg agtaccacct gggcattggc atcaccttca 781 tcgacatgag cgtgggcaac gtgaaggaag tggaccggcg cagcttcgac ctcaccacgc 841 cctaccgcat cttcagcttc tctgctgact cagagctaga gaaggagcag tggttggagg 901 ccatgcaggg agccatcgct gaggccctgt ctacctcgga ggtggccgag cgcatctggg 961 ctgcagcccc caacaggttc tgtgctgact gcggggctcc tcagcctgac tgggcctcca 1021 tcaacctctg tgttgttatc tgcaagcgct gtgcagggga gcaccgtggc ctgggcgctg 1081 gcgtctccaa ggtgcggagc ctgaagatgg acaggaaggt gtggacagaa acacttatcg 1141 aggccctgtg tgctgcagtc accaccacag acctggctga gacccaggcg ctcctgggct 1201 gtggggctgg gatcaactgc ttctcggggg accctgaggc ccccacgccc ctggctcttg 1261 cagagcaggc ggggcagacg ctgcagatgg aattccttcg gaacaaccgg accacagagg 1321 tgcctcggct ggactcgatg aagcccctgg aaaagcacta ctcagttgtc ctgccgaccg 1381 tgagccacag tggcttcctc tacaagactg cctctgccgg caagctgcta caggaccgcc 1441 gggcccggga agagttcagc cggcgctggt gtgtccttgg tgacggggtc ctgagctact 1501 ttgagaatga gcgggcagtg acccccaatg gagagattcg ggccagcgag attgtgtgcc 1561 tggcagtgcc ccctcctgac acccatggct ttgagcacac ctttgaggtg tacacggagg 1621 gagaacggct gtacctgttt gggctggaga gtgcggagca ggctcatgag tgggtcaagt 1681 gtattgctaa ggcattcgtg cctcccctag ccgaggatct gctggcccgg gattttgagc 1741 ggctgggacg cctaccctac aaagctggcc tgagcctaca gcgggcccag gagggctggt 1801 tctctctcag tggctcggag ctccgtgctg tcttcccgga ggggccctgc gaagagccgc 1861 tgcaactacg gaaactgcag gagctttcca tccaggggga cagtgagaac caggtgctgg 1921 tgctagtgga gcgaaggagg acactgtaca tacagggcga gcggcggctg gacttcatgg 1981 gttggctggg ggccatccag aaagcagccg ccagcatggg ggacacgctg tcggagcagc 2041 agcttgggga ctcggatatc ccggtgatcg tgtaccgctg tgtggactac atcacgcagt 2101 gcggcctgac ctccgagggc atctaccgca agtgtgggca gacatcgaag acacagcggc 2161 tgctggagag cctgcggcag gatgcgcgct ctgtgcacct caaggagggc gagcagcacg 2221 tggatgatgt ttcctcggcg ctcaagcgct tcctgcgcga cctgcctgat gggctcttca 2281 ctcgcgccca gcgcctaacc tggctggagg cctcagagat tgaggacgag gaggagaagg 2341 tctccaggta ccgagagctg ctggtgcggc tgccccctgt caaccgggcc acagtgaagg 2401 cccttatcag ccacctgtac tgtgttcagt gcttctcaga cacgaaccag atgaacgtgc 2461 acaacctggc aattgtgttt gggcccacgc tcttccagac agatgggcag gactacaagg 2521 ctggccgtgt ggtggaagac ctcattaacc actatgtggt ggtgtttagt gtggatgagg 2581 aagagctcag gaagcagcgg gaggagatca ctgccattgt gaagatgcgc gtggctggca 2641 ctgccagtgg gacccagcat gccggtgact tcatctgcac agtgtatctg gaagagaaga 2701 aggcagagac tgagcagcat atcaaggtcc cagcatccat gactgctgag gagctcaccc 2761 tggagatcct ggatcgccgg aacgtgggca tcagggagaa ggactattgg acctgctttg 2821 aggtcaacga gagggaggag gcagagcgcc ccctgcactt tgcggagaag gtgctgccca 2881 tcctgcacgg gctgggcacg gacagccacc tggtggtgaa gaagcaccag gccatggagg 2941 ccatgctgct gtacctggcc agccgtgtcg gtgacaccaa gcatggcatg atgaagttcc 3001 gtgaggaccg cagcctcctg ggcctgggcc tgccctcagg tggcttccac gatcgctact 3061 tcatcctcaa cagcagctgc ttgcggctct acaaggaggt ccggagtcac cggccTGAGA 3121 AGGAGTGGCC TATTAAGAGT CTCAAAGTCT ACCTGGGAGT GAAGAAGAAA CTCAGGCCAC 3181 CCACCTGCTG GGGCTTCACA GTGGTGCATG AGACAGAGAA ACATGAGAAG CAGCAGTGGT 3241 ACCTCTGCTG TGACACACAG ATGGAGCTCC GGGAGTGGTT CGCTACCTTT CTGTTTGTGC 3301 AGCATGACGG CCTGGTGTGG CCCTCAGAGC CCTCACGCGT GTCCCGGGCA GTGCCTGAGG 3361 TCCGGCTGGG TAGTGTGTCA CTGATCCCCC TTCGAGGTAG TGAAAATGAA ATGCGCCGGA 3421 GTGTGGCTGC CTTCACCGCG GACCCTCTGT CTCTTCTGCG CAACGTCTTG CCAACTTTCT 3481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3601 GTGGAAAGGA CGAAAATAAC GGCTCGCGCA CATTAATACG CGTTAAGTCg acaatcaacc 3661 tctggattac aaaatttgtg aaagatt