Transcript: Mouse NM_001199205.1

Mus musculus sperm associated antigen 9 (Spag9), transcript variant 7, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Spag9 (70834)
Length:
6940
CDS:
161..3694

Additional Resources:

NCBI RefSeq record:
NM_001199205.1
NBCI Gene record:
Spag9 (70834)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001199205.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000217384 GATAGGAAGTGCAGGTTAAAT pLKO.1 6118 3UTR 100% 15.000 21.000 N Spag9 n/a
2 TRCN0000215316 CACTAATAACAGACGGATTAT pLKO.1 4132 3UTR 100% 13.200 18.480 N Spag9 n/a
3 TRCN0000197843 GTCCTATATCATTAGGGATTT pLKO.1 330 CDS 100% 10.800 15.120 N Spag9 n/a
4 TRCN0000177286 GCTCTTCAAGTAATGCAACAA pLKO.1 1371 CDS 100% 4.950 6.930 N Spag9 n/a
5 TRCN0000177089 CCAGTGTTTCAGTCAAGTAAT pLKO.1 2486 CDS 100% 13.200 10.560 N Spag9 n/a
6 TRCN0000197596 CTCTTCAAGTAATGCAACAAA pLKO.1 1372 CDS 100% 5.625 3.938 N Spag9 n/a
7 TRCN0000177582 CCTGAATTGGATATGGACAAA pLKO.1 734 CDS 100% 4.950 3.465 N Spag9 n/a
8 TRCN0000176696 CCACATAATAATGTGAGCATT pLKO.1 3794 3UTR 100% 0.495 0.347 N Spag9 n/a
9 TRCN0000148340 CCAGGGACATTTATACCCTAT pLKO.1 3335 CDS 100% 4.050 2.835 N SPAG9 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001199205.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11327 pDONR223 100% 10% 9% None (many diffs) n/a
2 ccsbBroad304_11327 pLX_304 0% 10% 9% V5 (many diffs) n/a
3 TRCN0000479460 CGCTGAGTTCATAGCAAGTCTTAT pLX_317 87.6% 10% 9% V5 (many diffs) n/a
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