Transcript: Mouse XM_006535516.1

PREDICTED: Mus musculus DnaJ heat shock protein family (Hsp40) member C5 beta (Dnajc5b), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dnajc5b (66326)
Length:
1177
CDS:
357..956

Additional Resources:

NCBI RefSeq record:
XM_006535516.1
NBCI Gene record:
Dnajc5b (66326)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006535516.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000115365 CGACACCATCCAGACAAGAAT pLKO.1 489 CDS 100% 5.625 7.875 N Dnajc5b n/a
2 TRCN0000115362 GCAACTGTTGTTGTGGACATT pLKO.1 754 CDS 100% 4.950 6.930 N Dnajc5b n/a
3 TRCN0000115364 CCAGCCTACAAATACAAATGA pLKO.1 884 CDS 100% 5.625 3.938 N Dnajc5b n/a
4 TRCN0000115363 CTGAACAATTTGGAGATGAAA pLKO.1 628 CDS 100% 5.625 3.938 N Dnajc5b n/a
5 TRCN0000115361 GCTGTGAACATATTCACATTT pLKO.1 1041 3UTR 100% 1.320 0.924 N Dnajc5b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006535516.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04486 pDONR223 100% 89.6% 88.9% None (many diffs) n/a
2 ccsbBroad304_04486 pLX_304 0% 89.6% 88.9% V5 (many diffs) n/a
3 TRCN0000472112 GCCAGTCCGACTAGCATGACGTAC pLX_317 77.4% 89.6% 88.9% V5 (many diffs) n/a
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