Transcript: Human NM_001286489.1

Homo sapiens transmembrane protein 14B (TMEM14B), transcript variant 5, mRNA.

Source:
NCBI, updated 2018-07-01
Taxon:
Homo sapiens (human)
Gene:
TMEM14B (81853)
Length:
1209
CDS:
168..521

Additional Resources:

NCBI RefSeq record:
NM_001286489.1
NBCI Gene record:
TMEM14B (81853)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001286489.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000004949 AGCCGCTACATCTGTTACTTT pLKO.1 266 CDS 100% 5.625 7.875 N TMEM14B n/a
2 TRCN0000004950 GAGATCCTACTACTATGGAAA pLKO.1 305 CDS 100% 4.950 6.930 N TMEM14B n/a
3 TRCN0000368943 CTACACAGCACTGGTTGTTTC pLKO_005 218 CDS 100% 10.800 7.560 N TMEM14B n/a
4 TRCN0000364338 GAATGAGATCCTACTACTATG pLKO_005 301 CDS 100% 10.800 7.560 N TMEM14B n/a
5 TRCN0000364464 ATTCATGCCTGTAGGTTTAAT pLKO_005 326 CDS 100% 15.000 9.000 N TMEM14B n/a
6 TRCN0000364337 TGGGATCGTTGGCTATGTAAA pLKO_005 242 CDS 100% 13.200 7.920 N TMEM14B n/a
7 TRCN0000204533 CCTCCCAAAGTGCTGGAATTA pLKO.1 590 3UTR 100% 13.200 6.600 Y LRRC74B n/a
8 TRCN0000004951 GCCTTTGCATTGGTTTGGCTT pLKO.1 194 CDS 100% 2.640 1.320 Y TMEM14B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001286489.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16015 pDONR223 0% 49.1% 45% None (many diffs) n/a
2 ccsbBroad304_16015 pLX_304 0% 49.1% 45% V5 (many diffs) n/a
3 TRCN0000465833 AACACTTAGCGCTCAAGGTAGGTC pLX_317 74.3% 48.7% 45% V5 (many diffs) n/a
4 ccsbBroadEn_04259 pDONR223 100% 49.1% 45% None (many diffs) n/a
5 ccsbBroad304_04259 pLX_304 0% 49.1% 45% V5 (many diffs) n/a
6 TRCN0000465648 CAGCGGCAAACTCAGCTGAACCGA pLX_317 74.3% 49.1% 45% V5 (many diffs) n/a
7 ccsbBroadEn_09090 pDONR223 100% 48.8% 45% None (many diffs) n/a
8 ccsbBroad304_09090 pLX_304 0% 48.8% 45% V5 (many diffs) n/a
9 TRCN0000466087 GGTCCATACGCCGCTCTGTGTCCA pLX_317 74.3% 48.8% 45% V5 (many diffs) n/a
10 ccsbBroadEn_09089 pDONR223 100% 48.8% 44.3% None (many diffs) n/a
11 ccsbBroad304_09089 pLX_304 0% 48.8% 44.3% V5 (many diffs) n/a
12 TRCN0000470596 GAACCAGTCCTCTACACTAACCAT pLX_317 78.4% 48.8% 44.3% V5 (many diffs) n/a
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