Transcript: Mouse XM_006521292.2

PREDICTED: Mus musculus YY1 associated factor 2 (Yaf2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Yaf2 (67057)
Length:
2244
CDS:
137..748

Additional Resources:

NCBI RefSeq record:
XM_006521292.2
NBCI Gene record:
Yaf2 (67057)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006521292.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000095204 GCAGTGTTATTGGTAAGTCTA pLKO.1 988 3UTR 100% 4.950 6.930 N Yaf2 n/a
2 TRCN0000332379 GCAGTGTTATTGGTAAGTCTA pLKO_005 988 3UTR 100% 4.950 6.930 N Yaf2 n/a
3 TRCN0000108028 CGAGGCCTTCAAGTGCATGAT pLKO.1 238 CDS 100% 4.950 3.960 N YAF2 n/a
4 TRCN0000095208 CAAGTGCATGATGTGCGATGT pLKO.1 247 CDS 100% 4.050 3.240 N Yaf2 n/a
5 TRCN0000332306 CAAGTGCATGATGTGCGATGT pLKO_005 247 CDS 100% 4.050 3.240 N Yaf2 n/a
6 TRCN0000095207 CAGTCGAAGAAAGAGAAGAAA pLKO.1 425 CDS 100% 5.625 3.938 N Yaf2 n/a
7 TRCN0000095205 CCAGTCGAAGAAAGAGAAGAA pLKO.1 424 CDS 100% 4.950 3.465 N Yaf2 n/a
8 TRCN0000332381 CCAGTCGAAGAAAGAGAAGAA pLKO_005 424 CDS 100% 4.950 3.465 N Yaf2 n/a
9 TRCN0000095206 GTTGGAGATCTAACAGTCATT pLKO.1 566 CDS 100% 4.950 3.465 N Yaf2 n/a
10 TRCN0000332380 GTTGGAGATCTAACAGTCATT pLKO_005 566 CDS 100% 4.950 3.465 N Yaf2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006521292.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07561 pDONR223 100% 84.3% 87.2% None (many diffs) n/a
2 ccsbBroad304_07561 pLX_304 0% 84.3% 87.2% V5 (many diffs) n/a
3 TRCN0000470372 CGGTATTACTATGAGTGCGCAACC pLX_317 77.3% 84.3% 87.2% V5 (many diffs) n/a
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