Transcript: Mouse NM_025697.4

Mus musculus SPRY domain containing 7 (Spryd7), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Spryd7 (66674)
Length:
4144
CDS:
261..851

Additional Resources:

NCBI RefSeq record:
NM_025697.4
NBCI Gene record:
Spryd7 (66674)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025697.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000191304 CCTCTGTTAATGGACTACTTT pLKO.1 1296 3UTR 100% 5.625 7.875 N Spryd7 n/a
2 TRCN0000314425 CCTCTGTTAATGGACTACTTT pLKO_005 1296 3UTR 100% 5.625 7.875 N Spryd7 n/a
3 TRCN0000191481 GCTGATCTATTTCCTCTGTTA pLKO.1 1284 3UTR 100% 4.950 3.465 N Spryd7 n/a
4 TRCN0000314424 GCTGATCTATTTCCTCTGTTA pLKO_005 1284 3UTR 100% 4.950 3.465 N Spryd7 n/a
5 TRCN0000191760 GAAAGTTAACTTGAACCAGAT pLKO.1 512 CDS 100% 4.050 2.835 N Spryd7 n/a
6 TRCN0000314423 GAAAGTTAACTTGAACCAGAT pLKO_005 512 CDS 100% 4.050 2.835 N Spryd7 n/a
7 TRCN0000189913 GATGAGAAATGACGGAGCCTT pLKO.1 563 CDS 100% 2.640 1.848 N Spryd7 n/a
8 TRCN0000350326 GATGAGAAATGACGGAGCCTT pLKO_005 563 CDS 100% 2.640 1.848 N Spryd7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025697.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08717 pDONR223 100% 72.9% 78% None (many diffs) n/a
2 TRCN0000470473 TTCATAAACATATGGGTGTCTATC pLX_317 85.5% 72.9% 78% V5 (many diffs) n/a
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