Transcript: Mouse NM_030704.3

Mus musculus heat shock protein 8 (Hspb8), mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Hspb8 (80888)
Length:
1825
CDS:
386..976

Additional Resources:

NCBI RefSeq record:
NM_030704.3
NBCI Gene record:
Hspb8 (80888)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_030704.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000088559 CCACTGTATTTGCCTCGCTTT pLKO.1 840 CDS 100% 4.050 5.670 N Hspb8 n/a
2 TRCN0000088562 GTGGAAGTTTCAGGCAAACAT pLKO.1 740 CDS 100% 5.625 3.938 N Hspb8 n/a
3 TRCN0000302431 GTGGAAGTTTCAGGCAAACAT pLKO_005 740 CDS 100% 5.625 3.938 N Hspb8 n/a
4 TRCN0000088560 GCCTTGGAAAGTGTGTGTCAA pLKO.1 667 CDS 100% 4.950 3.465 N Hspb8 n/a
5 TRCN0000302430 GCCTTGGAAAGTGTGTGTCAA pLKO_005 667 CDS 100% 4.950 3.465 N Hspb8 n/a
6 TRCN0000088558 GCTTGGTTGATGGGACTCATT pLKO.1 1373 3UTR 100% 4.950 3.465 N Hspb8 n/a
7 TRCN0000302490 GCTTGGTTGATGGGACTCATT pLKO_005 1373 3UTR 100% 4.950 3.465 N Hspb8 n/a
8 TRCN0000088561 TGGGATTGTCTCCAAGAACTT pLKO.1 781 CDS 100% 4.950 3.465 N Hspb8 n/a
9 TRCN0000302492 TGGGATTGTCTCCAAGAACTT pLKO_005 781 CDS 100% 4.950 3.465 N Hspb8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030704.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02958 pDONR223 100% 87.4% 94.3% None (many diffs) n/a
2 ccsbBroad304_02958 pLX_304 0% 87.4% 94.3% V5 (many diffs) n/a
3 TRCN0000465326 GCCTATTAACACACTAGCACTATA pLX_317 51.3% 87.4% 94.3% V5 (many diffs) n/a
4 ccsbBroadEn_15034 pDONR223 0% 87.4% 94.3% None (many diffs) n/a
5 ccsbBroad304_15034 pLX_304 0% 87.4% 94.3% V5 (many diffs) n/a
6 TRCN0000479643 AGAGGAATTTTACTATTGCCGGAT pLX_317 54.1% 87.4% 94.3% V5 (many diffs) n/a
7 TRCN0000491253 GCAGATTCCGCATCTGCAATCATT pLX_317 41.1% 87.4% 94.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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