Transcript: Human XM_005263656.4

PREDICTED: Homo sapiens speckle type BTB/POZ protein like (SPOPL), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SPOPL (339745)
Length:
5626
CDS:
518..1471

Additional Resources:

NCBI RefSeq record:
XM_005263656.4
NBCI Gene record:
SPOPL (339745)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005263656.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000141108 CGACAACTTGGGTGTAAAGAT pLKO.1 1286 CDS 100% 5.625 7.875 N SPOPL n/a
2 TRCN0000122565 CGGCTGAAGGTCATGTGCGAA pLKO.1 1142 CDS 100% 0.880 1.232 N SPOPL n/a
3 TRCN0000142235 GCAACCGACATAATGGAAACA pLKO.1 1331 CDS 100% 4.950 3.960 N SPOPL n/a
4 TRCN0000145576 CCAGATGACAAGCTTACATTA pLKO.1 646 CDS 100% 13.200 9.240 N SPOPL n/a
5 TRCN0000144171 CAACCGACATAATGGAAACAT pLKO.1 1332 CDS 100% 5.625 3.938 N SPOPL n/a
6 TRCN0000121605 CCTGTCTTCATTACTTTCTAT pLKO.1 3279 3UTR 100% 5.625 3.938 N SPOPL n/a
7 TRCN0000144048 CCTTGAATAGTCATGTGTCAT pLKO.1 4324 3UTR 100% 4.950 3.465 N SPOPL n/a
8 TRCN0000098568 GCTGGTGTTACACACAGGTTA pLKO.1 328 5UTR 100% 4.950 3.465 N Spopl n/a
9 TRCN0000140284 GCTGGTGTTACACACAGGTTA pLKO.1 328 5UTR 100% 4.950 3.465 N SPOPL n/a
10 TRCN0000140307 CAGTTTGGCATTCCACGCAAA pLKO.1 1433 CDS 100% 4.050 2.835 N SPOPL n/a
11 TRCN0000098567 GCAGACAAATATGCACTGGAA pLKO.1 1121 CDS 100% 2.640 1.848 N Spopl n/a
12 TRCN0000121974 CCAGATTCATTTGGATTCATA pLKO.1 3186 3UTR 100% 5.625 3.375 N SPOPL n/a
13 TRCN0000122773 CAGTTGAAAGCACAAGCCATA pLKO.1 1238 CDS 100% 4.050 2.430 N SPOPL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005263656.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05463 pDONR223 100% 67% 57.1% None 0_1ins322;157_253del n/a
2 ccsbBroad304_05463 pLX_304 0% 67% 57.1% V5 0_1ins322;157_253del n/a
3 TRCN0000468146 AATGCAGGGCAGGAAACACACTCG pLX_317 30.6% 67% 57.1% V5 0_1ins322;157_253del n/a
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