Transcript: Human XM_017010729.1

PREDICTED: Homo sapiens family with sequence similarity 50 member B (FAM50B), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM50B (26240)
Length:
1636
CDS:
128..1105

Additional Resources:

NCBI RefSeq record:
XM_017010729.1
NBCI Gene record:
FAM50B (26240)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017010729.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000148360 CCCACTGTCAGACAGAAATTA pLKO.1 1361 3UTR 100% 15.000 10.500 N FAM50B n/a
2 TRCN0000148452 CCACAGTGACTGAGTTTGATT pLKO.1 1432 3UTR 100% 5.625 3.938 N FAM50B n/a
3 TRCN0000146439 CACCTTCTACGACTTCATCAT pLKO.1 871 CDS 100% 4.950 2.970 N FAM50B n/a
4 TRCN0000148395 CGAGAAGAACAAGCACATCTT pLKO.1 1021 CDS 100% 4.950 2.475 Y FAM50B n/a
5 TRCN0000149289 GTACGAGAAGAACAAGCACAT pLKO.1 1018 CDS 100% 4.050 2.025 Y FAM50B n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017010729.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02937 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02937 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468713 GCCTTATTAATCTTCCATGACCTC pLX_317 35% 100% 100% V5 n/a
Download CSV