Transcript: Human XM_017026289.1

PREDICTED: Homo sapiens calponin 1 (CNN1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CNN1 (1264)
Length:
1805
CDS:
186..1277

Additional Resources:

NCBI RefSeq record:
XM_017026289.1
NBCI Gene record:
CNN1 (1264)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017026289.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000426285 GAGAAGGGCGGAACATCATTG pLKO_005 856 CDS 100% 10.800 15.120 N CNN1 n/a
2 TRCN0000432726 GTGCTACAGGGTCCAACATAG pLKO_005 1475 3UTR 100% 10.800 15.120 N CNN1 n/a
3 TRCN0000421921 AGATCAATGAGTCAACCCAAA pLKO_005 604 CDS 100% 4.050 5.670 N CNN1 n/a
4 TRCN0000179672 GAAGATCAATGAGTCAACCCA pLKO.1 602 CDS 100% 0.750 1.050 N CNN1 n/a
5 TRCN0000417692 ACTTCATGGACGGCCTCAAAG pLKO_005 529 CDS 100% 10.800 7.560 N CNN1 n/a
6 TRCN0000430721 GACGCACTGAGCAACGCTATT pLKO_005 1524 3UTR 100% 10.800 7.560 N CNN1 n/a
7 TRCN0000155868 CATGGCGAAGACGAAAGGAAA pLKO.1 770 CDS 100% 4.950 3.465 N CNN1 n/a
8 TRCN0000417997 GGGAGTGAAGTACGCAGAGAA pLKO_005 803 CDS 100% 4.950 3.465 N CNN1 n/a
9 TRCN0000413481 TTGAGAACACCAACCATACAC pLKO_005 718 CDS 100% 4.950 3.465 N CNN1 n/a
10 TRCN0000179389 GACGAAAGGAAACAAGGTGAA pLKO.1 779 CDS 100% 4.050 2.835 N CNN1 n/a
11 TRCN0000154401 GAACTCAACCTCTACAGGGTT pLKO.1 1391 3UTR 100% 2.640 1.848 N CNN1 n/a
12 TRCN0000178949 CAACTACTACAATTCCGCCTA pLKO.1 1256 CDS 100% 2.160 1.512 N CNN1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017026289.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00338 pDONR223 100% 80% 75.6% None (many diffs) n/a
2 ccsbBroad304_00338 pLX_304 0% 80% 75.6% V5 (many diffs) n/a
3 TRCN0000473994 TTGCCTACAGGTCACGACGCCCAG pLX_317 58.3% 80% 75.6% V5 (many diffs) n/a
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