Transcript: Human XM_017017930.1

PREDICTED: Homo sapiens sialic acid acetylesterase (SIAE), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SIAE (54414)
Length:
5329
CDS:
3267..4265

Additional Resources:

NCBI RefSeq record:
XM_017017930.1
NBCI Gene record:
SIAE (54414)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017017930.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048928 GCTGCTCAATCTCACATATTA pLKO.1 3950 CDS 100% 15.000 21.000 N SIAE n/a
2 TRCN0000373536 ATATGGCCGTAGACGTCTTTA pLKO_005 4744 3UTR 100% 13.200 18.480 N SIAE n/a
3 TRCN0000373538 CCGACAGAAGTGCAGGTATTG pLKO_005 67 5UTR 100% 10.800 15.120 N SIAE n/a
4 TRCN0000048931 GCTTTGCTTCATACATCAATA pLKO.1 94 5UTR 100% 13.200 9.240 N SIAE n/a
5 TRCN0000373537 CATCGAAGACTGGCGTGAAAC pLKO_005 3581 CDS 100% 10.800 7.560 N SIAE n/a
6 TRCN0000048932 CCCTCCCTTCATTGCTTTCAT pLKO.1 4202 CDS 100% 5.625 3.938 N SIAE n/a
7 TRCN0000048929 CCATTCCATACGATTCTGTAA pLKO.1 3418 CDS 100% 4.950 3.465 N SIAE n/a
8 TRCN0000048930 CCCAATACTTTCATGGCTGTA pLKO.1 3759 CDS 100% 4.050 2.835 N SIAE n/a
9 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1113 5UTR 100% 5.625 2.813 Y KLHL30 n/a
10 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1113 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017017930.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08372 pDONR223 100% 63.4% 63.4% None 0_1ins573;942C>A n/a
2 ccsbBroad304_08372 pLX_304 0% 63.4% 63.4% V5 0_1ins573;942C>A n/a
3 TRCN0000473422 AGTACGAAAGAACTTCTCCCTATT pLX_317 29% 63.4% 63.4% V5 0_1ins573;942C>A n/a
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