Transcript: Mouse XM_006527853.3

PREDICTED: Mus musculus inhibitor of kappaB kinase gamma (Ikbkg), transcript variant X8, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ikbkg (16151)
Length:
9963
CDS:
295..1530

Additional Resources:

NCBI RefSeq record:
XM_006527853.3
NBCI Gene record:
Ikbkg (16151)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006527853.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000088812 AGACTACGACAGCCACATTAA pLKO.1 1008 CDS 100% 13.200 9.240 N Ikbkg n/a
2 TRCN0000302309 AGACTACGACAGCCACATTAA pLKO_005 1008 CDS 100% 13.200 9.240 N Ikbkg n/a
3 TRCN0000088811 GTAGCCAAACAGGAATTGATT pLKO.1 1093 CDS 100% 5.625 3.938 N Ikbkg n/a
4 TRCN0000302306 GTAGCCAAACAGGAATTGATT pLKO_005 1093 CDS 100% 5.625 3.938 N Ikbkg n/a
5 TRCN0000088808 CCACACTTAAGGGCTTGCTTT pLKO.1 1609 3UTR 100% 4.950 3.465 N Ikbkg n/a
6 TRCN0000302372 CCACACTTAAGGGCTTGCTTT pLKO_005 1609 3UTR 100% 4.950 3.465 N Ikbkg n/a
7 TRCN0000088809 GCTCCTGATATGGACACTCTA pLKO.1 1480 CDS 100% 4.950 3.465 N Ikbkg n/a
8 TRCN0000022146 GCAGCACAAGATTGTGATGGA pLKO.1 1137 CDS 100% 2.640 1.848 N IKBKG n/a
9 TRCN0000088810 GCCTTAAAGGAGTTGGAGCAA pLKO.1 655 CDS 100% 2.640 1.848 N Ikbkg n/a
10 TRCN0000302371 GCCTTAAAGGAGTTGGAGCAA pLKO_005 655 CDS 100% 2.640 1.848 N Ikbkg n/a
11 TRCN0000022147 GAGAATCAAGAGCTCCGAGAT pLKO.1 463 CDS 100% 4.050 2.835 N IKBKG n/a
12 TRCN0000166364 CACACACACACACACACACAA pLKO.1 9334 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006527853.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01945 pDONR223 100% 83.3% 86.3% None (many diffs) n/a
2 ccsbBroad304_01945 pLX_304 16.3% 83.3% 86.3% V5 (many diffs) n/a
3 TRCN0000472009 CACGCCGCGCATTCCGTCTACAAC pLX_317 35% 83.3% 86.3% V5 (many diffs) n/a
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