Construct: ORF TRCN0000472009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006817.1_s317c1
- Derived from:
- ccsbBroadEn_01945
- DNA Barcode:
- CACGCCGCGCATTCCGTCTACAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IKBKG (8517)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472009
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8517 | IKBKG | inhibitor of nuclear factor... | NM_001099857.3 | 100% | 100% | |
| 2 | human | 8517 | IKBKG | inhibitor of nuclear factor... | NM_001321396.2 | 100% | 100% | |
| 3 | human | 8517 | IKBKG | inhibitor of nuclear factor... | NM_003639.4 | 100% | 100% | |
| 4 | human | 8517 | IKBKG | inhibitor of nuclear factor... | NM_001321397.2 | 99.7% | 99.7% | 393_394insCAG |
| 5 | human | 8517 | IKBKG | inhibitor of nuclear factor... | NM_001099856.5 | 86% | 86% | 1_204del |
| 6 | human | 8517 | IKBKG | inhibitor of nuclear factor... | NM_001145255.3 | 76.3% | 76.3% | 518_519ins153;615_616ins144 |
| 7 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | NM_001161423.1 | 83.6% | 86.6% | (many diffs) |
| 8 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | NM_010547.2 | 83.6% | 86.6% | (many diffs) |
| 9 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | XM_006527850.2 | 83.6% | 86.6% | (many diffs) |
| 10 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | XM_006527851.2 | 83.6% | 86.6% | (many diffs) |
| 11 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | XM_006527852.2 | 83.6% | 86.6% | (many diffs) |
| 12 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | XM_011247527.2 | 83.6% | 86.6% | (many diffs) |
| 13 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | NM_001161424.1 | 83.3% | 86.3% | (many diffs) |
| 14 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | NM_178590.4 | 83.3% | 86.3% | (many diffs) |
| 15 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | XM_006527853.3 | 83.3% | 86.3% | (many diffs) |
| 16 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | XM_006527849.2 | 82.4% | 85.4% | (many diffs) |
| 17 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | NM_001161422.1 | 81.2% | 84.2% | (many diffs) |
| 18 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | NM_001136067.2 | 80.1% | 83% | (many diffs) |
| 19 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | XM_006527847.3 | 80.1% | 83% | (many diffs) |
| 20 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | XM_006527848.3 | 80.1% | 83% | (many diffs) |
| 21 | mouse | 16151 | Ikbkg | inhibitor of kappaB kinase ... | NM_001161421.1 | 79.9% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1323
- ORF length:
- 1257
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa taggcacctc tggaagagcc aactgtgtga gatggtgcag cccagtggtg 121 gcccggcagc agatcaggac gtactgggcg aagagtctcc tctggggaag ccagccatgc 181 tgcacctgcc ttcagaacag ggcgctcctg agaccctcca gcgctgcctg gaggagaatc 241 aagagctccg agatgccatc cggcagagca accagattct gcgggagcgc tgcgaggagc 301 ttctgcattt ccaagccagc cagagggagg agaaggagtt cctcatgtgc aagttccagg 361 aggccaggaa actggtggag agactcggcc tggagaagct cgatctgaag aggcagaagg 421 agcaggctct gcgggaggtg gagcacctga agagatgcca gcagcagatg gctgaggaca 481 aggcctctgt gaaagcccag gtgacgtcct tgctcgggga gctgcaggag agccagagtc 541 gcttggaggc tgccactaag gaatgccagg ctctggaggg tcgggcccgg gcggccagcg 601 agcaggcgcg gcagctggag agtgagcgcg aggcgctgca gcagcagcac agcgtgcagg 661 tggaccagct gcgcatgcag ggccagagcg tggaggccgc gctccgcatg gagcgccagg 721 ccgcctcgga ggagaagagg aagctggccc agttgcaggt ggcctatcac cagctcttcc 781 aagaatacga caaccacatc aagagcagcg tggtgggcag tgagcggaag cgaggaatgc 841 agctggaaga tctcaaacag cagctccagc aggccgagga ggcccTGGTG GCCAAACAGG 901 AGGTGATCGA TAAGCTGAAG GAGGAGGCCG AGCAGCACAA GATTGTGATG GAGACCGTTC 961 CGGTGCTGAA GGCCCAGGCG GATATCTACA AGGCGGACTT CCAGGCTGAG AGGCAGGCCC 1021 GGGAGAAGCT GGCCGAGAAG AAGGAGCTCC TGCAGGAGCA GCTGGAGCAG CTGCAGAGGG 1081 AGTACAGCAA ACTGAAGGCC AGCTGTCAGG AGTCGGCCAG GATCGAGGAC ATGAGGAAGC 1141 GGCATGTCGA GGTCTCCCAG GCCCCCTTGC CCCCCGCCCC TGCCTACCTC TCCTCTCCCC 1201 TGGCCCTGCC CAGCCAGAGG AGGAGCCCCC CCGAGGAGCC ACCTGACTTC TGCTGTCCCA 1261 AGTGCCAGTA TCAGGCCCCT GATATGGACA CCCTGCAGAT ACATGTCATG GAGTGCATTG 1321 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGACA CGCCGCGCAT TCCGTCTACA ACACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt