Transcript: Human NR_146221.2

Homo sapiens transmembrane and coiled-coil domains 3 (TMCO3), transcript variant 8, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
TMCO3 (55002)
Length:
2782
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_146221.2
NBCI Gene record:
TMCO3 (55002)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_146221.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000145276 GATTGCCTACAATGTTTGGTT pLKO.1 1208 3UTR 100% 3.000 4.200 N TMCO3 n/a
2 TRCN0000142731 CCGGAGTAGTTTATTTGCAGT pLKO.1 2273 3UTR 100% 2.640 3.696 N TMCO3 n/a
3 TRCN0000145112 GTCTGTTGATTATGTGCAGTA pLKO.1 2292 3UTR 100% 4.050 3.240 N TMCO3 n/a
4 TRCN0000144829 GTGGAGACATTAGGAGAATTT pLKO.1 1291 3UTR 100% 13.200 9.240 N TMCO3 n/a
5 TRCN0000140058 CGTGAGCTTGGAAGCTTACTT pLKO.1 2545 3UTR 100% 5.625 3.938 N TMCO3 n/a
6 TRCN0000140852 CTTGGGTCTTAGCATGCTGAT pLKO.1 1050 3UTR 100% 4.050 2.835 N TMCO3 n/a
7 TRCN0000143549 GAAGAGGCCAATTCTAAGCAA pLKO.1 1000 3UTR 100% 3.000 2.100 N TMCO3 n/a
8 TRCN0000140136 GATGTAAGAAAGGCAGCGGAT pLKO.1 889 3UTR 100% 2.160 1.512 N TMCO3 n/a
9 TRCN0000121682 GAGACAAATACGGATGTCTAT pLKO.1 2596 3UTR 100% 0.495 0.347 N TMCO3 n/a
10 TRCN0000144909 GCAGTTCTGAACAAACTGAAA pLKO.1 529 3UTR 100% 0.495 0.297 N TMCO3 n/a
11 TRCN0000200883 GACGACAATAAATCAGTCAAA pLKO.1 943 3UTR 100% 4.950 6.930 N Tmco3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_146221.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08447 pDONR223 100% 57.3% None (many diffs) n/a
2 ccsbBroad304_08447 pLX_304 0% 57.3% V5 (many diffs) n/a
3 TRCN0000491571 CTGATCTCAAAAAGCTGTAGGTAG pLX_317 12.8% 57.3% V5 (many diffs) n/a
4 ccsbBroadEn_12131 pDONR223 100% 44.6% None (many diffs) n/a
5 ccsbBroad304_12131 pLX_304 0% 44.6% V5 (many diffs) n/a
6 TRCN0000480630 ACAGAGATGTCACGTATCACGGAA pLX_317 29.4% 44.6% V5 (many diffs) n/a
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