Transcript: Human XM_005244715.2

PREDICTED: Homo sapiens ArfGAP with coiled-coil, ankyrin repeat and PH domains 3 (ACAP3), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACAP3 (116983)
Length:
3696
CDS:
111..2516

Additional Resources:

NCBI RefSeq record:
XM_005244715.2
NBCI Gene record:
ACAP3 (116983)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005244715.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000424659 ACTATGTGCTCCAGATCAATG pLKO_005 619 CDS 100% 10.800 15.120 N ACAP3 n/a
2 TRCN0000438324 TGATCGACTCTGCGGTGGAAA pLKO_005 787 CDS 100% 4.950 6.930 N ACAP3 n/a
3 TRCN0000155903 CAGAGCTTTGTCAAAGAGGAT pLKO.1 432 CDS 100% 2.640 3.696 N ACAP3 n/a
4 TRCN0000157528 GCAACGCTTTCAAGACATGGA pLKO.1 949 CDS 100% 2.640 2.112 N ACAP3 n/a
5 TRCN0000158197 CAAAGAGGATGTGCGGAAGTT pLKO.1 443 CDS 100% 4.950 3.465 N ACAP3 n/a
6 TRCN0000157741 CCAGCAACGCTTTCAAGACAT pLKO.1 946 CDS 100% 4.950 3.465 N ACAP3 n/a
7 TRCN0000424575 GCTCATGTCACAAGCCACTTT pLKO_005 2804 3UTR 100% 4.950 3.465 N ACAP3 n/a
8 TRCN0000156390 CGATGAGTCCAAAGTGGAGTT pLKO.1 872 CDS 100% 4.050 2.835 N ACAP3 n/a
9 TRCN0000437572 TGTGAAGCCGTGTGAGGACAT pLKO_005 1067 CDS 100% 4.050 2.835 N ACAP3 n/a
10 TRCN0000438876 CAACGCTGACATCGTGACACT pLKO_005 2351 CDS 100% 2.640 1.848 N ACAP3 n/a
11 TRCN0000437735 ACACCGTCATCTCGGAATGTC pLKO_005 322 CDS 100% 4.950 2.970 N ACAP3 n/a
12 TRCN0000156962 GCAGGCCAAGAAGAAGTTTGA pLKO.1 644 CDS 100% 4.950 2.970 N ACAP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005244715.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09438 pDONR223 100% 96% 95.8% None 281T>A;1813_1814ins99 n/a
2 ccsbBroad304_09438 pLX_304 0% 96% 95.8% V5 281T>A;1813_1814ins99 n/a
3 TRCN0000481075 CTGTTCATACGTCAGCGGCCGAGT pLX_317 18.4% 96% 95.8% V5 281T>A;1813_1814ins99 n/a
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