Construct: ORF TRCN0000481075
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015077.1_s317c1
- Derived from:
- ccsbBroadEn_09438
- DNA Barcode:
- CTGTTCATACGTCAGCGGCCGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACAP3 (116983)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481075
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | NM_030649.3 | 99.9% | 99.8% | 281T>A |
| 2 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | XM_011540606.2 | 98.7% | 98.6% | 225_254del;311T>A |
| 3 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | XM_024452993.1 | 97.6% | 97.2% | (many diffs) |
| 4 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | XM_011540607.1 | 96.5% | 96.1% | (many diffs) |
| 5 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | XM_005244715.2 | 96% | 95.8% | 281T>A;1813_1814ins99 |
| 6 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | XM_011540608.2 | 94.9% | 94.9% | 225_254del;311T>A;597_598ins96 |
| 7 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | XM_011540609.2 | 94.8% | 94.6% | 225_254del;311T>A;1843_1844ins99 |
| 8 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | XM_017000233.1 | 77.3% | 77.3% | 0_1ins567 |
| 9 | human | 116983 | ACAP3 | ArfGAP with coiled-coil, an... | XM_005244717.3 | 59.1% | 59.1% | 0_1ins1023 |
| 10 | mouse | 140500 | Acap3 | ArfGAP with coiled-coil, an... | NM_207223.1 | 85.7% | 93.6% | (many diffs) |
| 11 | mouse | 140500 | Acap3 | ArfGAP with coiled-coil, an... | XM_006538541.3 | 81.4% | 88.7% | (many diffs) |
| 12 | mouse | 140500 | Acap3 | ArfGAP with coiled-coil, an... | XM_006538542.1 | 81.4% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2571
- ORF length:
- 2502
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgtggag ttcgaggagt gcgtcaagga ctccccgcgc ttcagggcga 121 ccattgacga ggtggagacg gacgtggtgg agattgaggc caaactggac aagctggtga 181 agctgtgcag tggcatggtg gaagccggta aggcctacgt cagcaccagc aggcttttcg 241 tgagcggcgt ccgcgacctg tcccagcagt gccagggcga caccgtcatc tcggaatgtc 301 tgcagaggtt cgctgacagc ctacaggagg tggtgaacta ccacatgaac ctgtttgacc 361 aggcccagag gtccgtgcgg cagcagctcc agagctttgt caaagaggat gtgcggaagt 421 tcaaggagac aaagaagcag tttgacaagg tgcgggagga cctggagctg tccctggtga 481 ggaacgccca ggccccgagg caccggcccc acgaggtgga ggaagccacc ggggccctca 541 ccctcaccag gaagtgcttc cgccacctgg cactggacta tgtgctccag atcaatgttc 601 tgcaggccaa gaagaagttt gagatcctgg actctatgct gtccttcatg cacgcccagt 661 ccagcttctt ccagcagggc tacagcctcc tgcaccagct ggacccctac atgaagaagc 721 tggcagccga gctggaccag ctggtgatcg actctgcggt ggaaaagcgt gagatggagc 781 gaaagcacgc cgccatccag cagcggacgc tgctgcagga cttctcctac gatgagtcca 841 aagtggagtt tgacgtggac gcgcccagtg gggtggtgat ggagggctac ctcttcaaga 901 gggccagcaa cgctttcaag acatggaacc ggcgctggtt ctccattcag aacagccagc 961 tggtctacca gaagaagctc aaggatgccc tcaccgtggt ggtggatgac ctccgcctgt 1021 gctctgtgaa gccgtgtgag gacatcgagc ggaggttctg cttcgaggtg ctgtcaccca 1081 ccaagagctg catgctgcag gctgactccg agaagctgcg gcaagcctgg gtccaggctg 1141 tgcaggccag catcgcctcc gcctaccgcg agagccctga cagttgctat agcgagaggc 1201 tggaccgcac agcatccccg tccacgagca gcatcgactc cgccaccgac actcgggagc 1261 gtggcgtgaa gggcgagagt gtgctgcagc gtgtgcagag tgtggccggc aacagccagt 1321 gcggcgactg cggccagccg gacccccgct gggccagcat caacctgggc gtgctgctct 1381 gcattgagtg ctccggcatc cacaggagcc tgggtgtcca ctgctccaag gtgcggtccc 1441 tgacgctgga ctcgtgggag cctgagctgc taaagctgat gtgtgagctt ggaaacagcg 1501 ctgtgaatca gatctatgag gcccagtgtg agggtgcagg cagcaggaaa cccacagcca 1561 gcagctcccg gcaggacaag gaggcctgga tcaaggacaa atacgtggaa aagaagtttc 1621 tgcggaaggc gcccatggca ccagccctgg aggccccaag acgctggagg gtgcagaagt 1681 gcctgcggcc ccacagctct ccccgcgctc ccactgcccg ccgcaaggtc cggcttgagc 1741 ccgttctgcc ctgtgtggcc gctctgtcct cagtgggcac cctggatcgt aagttccgcc 1801 gagactccct cttctgtccc gacgagctgg actcgctctt ctcctacttc gacgcagggg 1861 ccgcaggggc tggccctcgc agtctgagta gcgacagtgg ccttgggggc agctcggatg 1921 gcagctcgga cgtcctggct ttcggctcgg gctctgtggt ggacagcgtc actgaggagg 1981 agggtgcaga gtcggaggag tccagcggtg aggcagacgg ggacactgag gccgaggcct 2041 ggggcctggc ggacgtgcgc gagctgcacc cggggctctt ggcgcaccgc gcagcgcgtg 2101 cccgcgacct tcctGCGCTG GCGGCGGCGC TGGCCCACGG GGCCGAGGTC AACTGGGCGG 2161 ACGCGGAGGA TGAGGGCAAG ACGCCGCTGG TGCAGGCCGT GCTAGGGGGC TCCTTGATCG 2221 TCTGTGAGTT CCTGCTGCAA AACGGAGCGG ACGTGAACCA AAGAGACAGC CGGGGCCGGG 2281 CGCCCCTGCA CCACGCCACG CTGCTGGGCC GCACCGGCCA GGTTTGCCTG TTCCTGAAGC 2341 GGGGCGCGGA CCAGCACGCC CTGGACCAAG AGCAGCGGGA CCCGTTGGCC ATCGCAGTGC 2401 AGGCGGCCAA CGCTGACATC GTGACACTGC TCCGTCTGGC GCGCATGGCG GAGGAAATGC 2461 GCGAGGCCGA GGCTGCCCCT GGTCCCCCGG GCGCCCTGGC GGGCAGCCCC ACGGAGCTCC 2521 AGTTCCGCAG GTGTATCCAG GAGTTCATCA GCCTCCACCT GGAAGAGAGC TTGCCAACTT 2581 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2641 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2701 CTTGTGGAAA GGACGACTGT TCATACGTCA GCGGCCGAGT ACGCGTTAAG TCgacaatca 2761 acctctggat tacaaaattt gtgaaagatt