Construct: ORF TRCN0000481075

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015077.1_s317c1
Derived from:
ccsbBroadEn_09438
DNA Barcode:
CTGTTCATACGTCAGCGGCCGAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACAP3 (116983)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481075
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 116983 ACAP3 ArfGAP with coiled-coil, an... NM_030649.3 99.9% 99.8% 281T>A
2 human 116983 ACAP3 ArfGAP with coiled-coil, an... XM_011540606.2 98.7% 98.6% 225_254del;311T>A
3 human 116983 ACAP3 ArfGAP with coiled-coil, an... XM_024452993.1 97.6% 97.2% (many diffs)
4 human 116983 ACAP3 ArfGAP with coiled-coil, an... XM_011540607.1 96.5% 96.1% (many diffs)
5 human 116983 ACAP3 ArfGAP with coiled-coil, an... XM_005244715.2 96% 95.8% 281T>A;1813_1814ins99
6 human 116983 ACAP3 ArfGAP with coiled-coil, an... XM_011540608.2 94.9% 94.9% 225_254del;311T>A;597_598ins96
7 human 116983 ACAP3 ArfGAP with coiled-coil, an... XM_011540609.2 94.8% 94.6% 225_254del;311T>A;1843_1844ins99
8 human 116983 ACAP3 ArfGAP with coiled-coil, an... XM_017000233.1 77.3% 77.3% 0_1ins567
9 human 116983 ACAP3 ArfGAP with coiled-coil, an... XM_005244717.3 59.1% 59.1% 0_1ins1023
10 mouse 140500 Acap3 ArfGAP with coiled-coil, an... NM_207223.1 85.7% 93.6% (many diffs)
11 mouse 140500 Acap3 ArfGAP with coiled-coil, an... XM_006538541.3 81.4% 88.7% (many diffs)
12 mouse 140500 Acap3 ArfGAP with coiled-coil, an... XM_006538542.1 81.4% 88.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2571
ORF length:
2502
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaccgtggag ttcgaggagt gcgtcaagga ctccccgcgc ttcagggcga
121ccattgacga ggtggagacg gacgtggtgg agattgaggc caaactggac aagctggtga
181agctgtgcag tggcatggtg gaagccggta aggcctacgt cagcaccagc aggcttttcg
241tgagcggcgt ccgcgacctg tcccagcagt gccagggcga caccgtcatc tcggaatgtc
301tgcagaggtt cgctgacagc ctacaggagg tggtgaacta ccacatgaac ctgtttgacc
361aggcccagag gtccgtgcgg cagcagctcc agagctttgt caaagaggat gtgcggaagt
421tcaaggagac aaagaagcag tttgacaagg tgcgggagga cctggagctg tccctggtga
481ggaacgccca ggccccgagg caccggcccc acgaggtgga ggaagccacc ggggccctca
541ccctcaccag gaagtgcttc cgccacctgg cactggacta tgtgctccag atcaatgttc
601tgcaggccaa gaagaagttt gagatcctgg actctatgct gtccttcatg cacgcccagt
661ccagcttctt ccagcagggc tacagcctcc tgcaccagct ggacccctac atgaagaagc
721tggcagccga gctggaccag ctggtgatcg actctgcggt ggaaaagcgt gagatggagc
781gaaagcacgc cgccatccag cagcggacgc tgctgcagga cttctcctac gatgagtcca
841aagtggagtt tgacgtggac gcgcccagtg gggtggtgat ggagggctac ctcttcaaga
901gggccagcaa cgctttcaag acatggaacc ggcgctggtt ctccattcag aacagccagc
961tggtctacca gaagaagctc aaggatgccc tcaccgtggt ggtggatgac ctccgcctgt
1021gctctgtgaa gccgtgtgag gacatcgagc ggaggttctg cttcgaggtg ctgtcaccca
1081ccaagagctg catgctgcag gctgactccg agaagctgcg gcaagcctgg gtccaggctg
1141tgcaggccag catcgcctcc gcctaccgcg agagccctga cagttgctat agcgagaggc
1201tggaccgcac agcatccccg tccacgagca gcatcgactc cgccaccgac actcgggagc
1261gtggcgtgaa gggcgagagt gtgctgcagc gtgtgcagag tgtggccggc aacagccagt
1321gcggcgactg cggccagccg gacccccgct gggccagcat caacctgggc gtgctgctct
1381gcattgagtg ctccggcatc cacaggagcc tgggtgtcca ctgctccaag gtgcggtccc
1441tgacgctgga ctcgtgggag cctgagctgc taaagctgat gtgtgagctt ggaaacagcg
1501ctgtgaatca gatctatgag gcccagtgtg agggtgcagg cagcaggaaa cccacagcca
1561gcagctcccg gcaggacaag gaggcctgga tcaaggacaa atacgtggaa aagaagtttc
1621tgcggaaggc gcccatggca ccagccctgg aggccccaag acgctggagg gtgcagaagt
1681gcctgcggcc ccacagctct ccccgcgctc ccactgcccg ccgcaaggtc cggcttgagc
1741ccgttctgcc ctgtgtggcc gctctgtcct cagtgggcac cctggatcgt aagttccgcc
1801gagactccct cttctgtccc gacgagctgg actcgctctt ctcctacttc gacgcagggg
1861ccgcaggggc tggccctcgc agtctgagta gcgacagtgg ccttgggggc agctcggatg
1921gcagctcgga cgtcctggct ttcggctcgg gctctgtggt ggacagcgtc actgaggagg
1981agggtgcaga gtcggaggag tccagcggtg aggcagacgg ggacactgag gccgaggcct
2041ggggcctggc ggacgtgcgc gagctgcacc cggggctctt ggcgcaccgc gcagcgcgtg
2101cccgcgacct tcctGCGCTG GCGGCGGCGC TGGCCCACGG GGCCGAGGTC AACTGGGCGG
2161ACGCGGAGGA TGAGGGCAAG ACGCCGCTGG TGCAGGCCGT GCTAGGGGGC TCCTTGATCG
2221TCTGTGAGTT CCTGCTGCAA AACGGAGCGG ACGTGAACCA AAGAGACAGC CGGGGCCGGG
2281CGCCCCTGCA CCACGCCACG CTGCTGGGCC GCACCGGCCA GGTTTGCCTG TTCCTGAAGC
2341GGGGCGCGGA CCAGCACGCC CTGGACCAAG AGCAGCGGGA CCCGTTGGCC ATCGCAGTGC
2401AGGCGGCCAA CGCTGACATC GTGACACTGC TCCGTCTGGC GCGCATGGCG GAGGAAATGC
2461GCGAGGCCGA GGCTGCCCCT GGTCCCCCGG GCGCCCTGGC GGGCAGCCCC ACGGAGCTCC
2521AGTTCCGCAG GTGTATCCAG GAGTTCATCA GCCTCCACCT GGAAGAGAGC TTGCCAACTT
2581TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2641CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2701CTTGTGGAAA GGACGACTGT TCATACGTCA GCGGCCGAGT ACGCGTTAAG TCgacaatca
2761acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)