Transcript: Human XM_005258520.2

PREDICTED: Homo sapiens chromosome 19 open reading frame 47 (C19orf47), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
C19orf47 (126526)
Length:
3712
CDS:
6..1271

Additional Resources:

NCBI RefSeq record:
XM_005258520.2
NBCI Gene record:
C19orf47 (126526)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005258520.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231830 ACACCACGACAGGGAGTAAAC pLKO_005 757 CDS 100% 10.800 15.120 N C19orf47 n/a
2 TRCN0000231832 GAGGTCAAGGTCACCATTAAG pLKO_005 1140 CDS 100% 13.200 10.560 N C19orf47 n/a
3 TRCN0000231831 AGCCGGAGTCCTTGTCTAAAG pLKO_005 1030 CDS 100% 10.800 8.640 N C19orf47 n/a
4 TRCN0000231829 GCATGCTGCTGGATCTCAATA pLKO_005 235 CDS 100% 13.200 9.240 N C19orf47 n/a
5 TRCN0000231833 TCTCTCCCTGTCTTCACTTTG pLKO_005 1747 3UTR 100% 10.800 6.480 N C19orf47 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005258520.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04816 pDONR223 100% 83.8% 83.8% None 1_201del;549_550insGCA n/a
2 ccsbBroad304_04816 pLX_304 0% 83.8% 83.8% V5 1_201del;549_550insGCA n/a
3 TRCN0000468059 GCCCTATACCGAAGATCCTGGTTA pLX_317 29.4% 83.8% 83.8% V5 1_201del;549_550insGCA n/a
4 ccsbBroadEn_16085 pDONR223 0% 66.3% 66.3% None 1_423del;549_550insGCA n/a
5 ccsbBroad304_16085 pLX_304 0% 66.3% 66.3% V5 1_423del;549_550insGCA n/a
6 TRCN0000473304 CTTGCCTGACGGCTTACTTCGTGG pLX_317 48.5% 66.3% 66.3% V5 1_423del;549_550insGCA n/a
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