Construct: ORF ccsbBroad304_08640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006358.1_s304c1, BRDN0000410407
- Derived from:
- ccsbBroadEn_08640
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF4ENIF1 (56478)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08640
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | NM_001164502.2 | 99.9% | 100% | 1704C>T |
| 2 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_005261686.3 | 82.2% | 82.2% | 297_785del;1477_1512del;2229C>T |
| 3 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_005261687.3 | 82.2% | 82.2% | 297_785del;1477_1512del;2229C>T |
| 4 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_005261688.3 | 82.2% | 82.2% | 297_785del;1477_1512del;2229C>T |
| 5 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_011530281.3 | 82.2% | 82.2% | 297_785del;1477_1512del;2229C>T |
| 6 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | NM_001164501.2 | 82.1% | 82.1% | (many diffs) |
| 7 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | NM_019843.4 | 82.1% | 82.1% | (many diffs) |
| 8 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_011530280.2 | 82.1% | 82.1% | (many diffs) |
| 9 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_017028864.2 | 82.1% | 82.1% | (many diffs) |
| 10 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_006724281.4 | 78.9% | 78.9% | (many diffs) |
| 11 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XM_017028863.2 | 78.8% | 78.8% | (many diffs) |
| 12 | human | 56478 | EIF4ENIF1 | eukaryotic translation init... | XR_001755264.2 | 38.2% | (many diffs) | |
| 13 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | NM_001166547.1 | 72.2% | 72.2% | (many diffs) |
| 14 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | NM_023743.2 | 72.2% | 72.2% | (many diffs) |
| 15 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_006514841.2 | 72.2% | 72.2% | (many diffs) |
| 16 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_006514842.3 | 72.2% | 72.2% | (many diffs) |
| 17 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_006514843.3 | 72.2% | 72.2% | (many diffs) |
| 18 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | NM_001166548.1 | 71.8% | 71.3% | (many diffs) |
| 19 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | NM_001166549.1 | 71.8% | 71.3% | (many diffs) |
| 20 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_006514844.2 | 71.8% | 71.3% | (many diffs) |
| 21 | mouse | 74203 | Eif4enif1 | eukaryotic translation init... | XM_017314806.1 | 71.8% | 71.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2499
- ORF length:
- 2433
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga taggagaagt atgggtgaaa cagaaagtgg agatgctttc cttgacctga 121 agaagcctcc tgcctccaaa tgcccccatc gctatacaaa agaagaactc ttggatataa 181 aagaactccc ccattccaaa cagaggcctt catgcctttc tgaaaaatat gacagtgatg 241 gtgtctggga ccctgagaag tggcatgcct ctctctaccc agcttcaggg cggagctcac 301 cagtggaaag tctgaagaaa gagttggata cagaccggcc ttccctggtg cgcaggatag 361 taggtatagt agagtgcaat ggaggagtgg ccgaagagga tgaagtggag gtcatccttg 421 cacaggagcc tgcggctgat caggaagtgc caagggatgc tgtcttgcct gagcagtccc 481 caggagactt tgactttaat gagttcttta accttgataa ggtgccatgc ttggcttcga 541 tgatagaaga tgttttggga gaagggtcag tctctgccag tcggttcagt aggtggttct 601 ctaacccgag cagatcagga agccgatcca gcagtcttgg gtcaacacca catgaagagc 661 tagagagact tgcaggtctg gagcaagcca tcctctctcc tggacagaac tcggggaatt 721 actttgctcc tataccattg gaagaccatg ctgaaaataa agtggatatt ttagaaatgc 781 tacagaaagc caaagtggat ttgaaacctc ttctttccag cctttctgca aataaagaaa 841 aacttaaaga aagctcacat tcaggggttg tgctttcagt ggaggaggta gaagcaggtc 901 tgaagggctt gaaggttgac cagcaagtga agaattcaac tcccttcatg gcagaacacc 961 tagaagagac cttgagtgcc gtaaccaaca atcgacaact gaagaaagac ggagacatga 1021 ctgcgttcaa caagctagtg agcacaatga agcgaaacct tgaaagccat ttgatgtccc 1081 ctgctgagat tccaggccag cctgtcccta agaacatcct gcaggaactt ctgggtcaac 1141 cagttcagag acctgcttct tccaatcttc tgagtggcct tatggggagc ttggagccta 1201 caacatcttt actgggccaa agagcaccct ctcctccctt gtcacaggtg tttcaaactc 1261 gagcagcctc agctgactac cttcgcccaa gaataccatc accaattggt ttcacaccag 1321 gaccacagca gctactcgga gatccattcc aaggcatgcg caaacccatg agccccatca 1381 cagcccagca gatgagccag ctggagttgc aacaggcagc tttagaaggg ctggccttgc 1441 cacatgacct tgctgtacag gcagcaaact tctaccagcc tggttttggc aaaccacagg 1501 tggacagaac cagagatgga ttcagaaaca ggcaacagcg agtgaccaag tcaccagcac 1561 ccgtgcatcg agggaattcc tcttcccctg cccctgctgc ctccatcaca agcatgcttt 1621 ctccttcctt tacccctacc tcagtgattc gtaagatgta cgagagcaaa gagaaaagca 1681 aggaggagcc agcatctgga aaagcagctc ttggtgacag taaagaggat actcagaagg 1741 ccagtgaaga aaacctcctg tcatccagtt ctgtacccag tgccgatcga gactcttctc 1801 ccactacaaa ttccaaactg tcagcattac agaggtcttc gtgttccacc ccactgtccc 1861 aggccaaccg ttacaccaaa gaacaagatt atcgacctaa agcaactggg agaaaaacac 1921 ccaccttggc atccccagtt cctacaacac cttttctccg ccctgtccac caagttcccc 1981 ttgtccccca tgtccctatg gttaggcctg ctcaccagct tcacccaggg ttggtacaga 2041 ggatgctggc ccagggagta catccacagc atcttccaag tttgctccaa actggtgtgc 2101 ttcctcctgg gatggacttg agtcatttac agggaatatc tggccccatc ctgggtcagc 2161 ccttttaccc tttacctgct gctagtcacc ctctcttaaa ccctcgtcct ggaacacctc 2221 tgcatctggc aatggtgcaa cagcagctac agcgctcagt tctgcatcct ccaggctctg 2281 gttcccatgc agcagctgtc agcgttcaga caacccctca gaacgtgccc agccggtcag 2341 gcctgcccca catgcactcc cagctggagc atcgccccag ccagaggagc agctcccctg 2401 tgggccttgc caaatggttt ggctcagatg tgctacagca acccctgccc tccatgcccg 2461 ccaaagttat cagtgtagat gaattggaat accgacagtg cccaactttc ttgtacaaag 2521 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2581 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta