Transcript: Human XM_005264332.4

PREDICTED: Homo sapiens mutS homolog 2 (MSH2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MSH2 (4436)
Length:
7145
CDS:
63..2987

Additional Resources:

NCBI RefSeq record:
XM_005264332.4
NBCI Gene record:
MSH2 (4436)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005264332.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039671 GCATGTAATAGAGTGTGCTAA pLKO.1 2576 CDS 100% 4.950 6.930 N MSH2 n/a
2 TRCN0000010384 ATTCATGTTGCAGAGCTTGCT pLKO.1 2544 CDS 100% 2.640 3.696 N MSH2 n/a
3 TRCN0000344496 ATTCATGTTGCAGAGCTTGCT pLKO_005 2544 CDS 100% 2.640 3.696 N MSH2 n/a
4 TRCN0000039670 GCCTTGCTGAATAAGTGTAAA pLKO.1 1044 CDS 100% 13.200 10.560 N MSH2 n/a
5 TRCN0000332891 GCCTTGCTGAATAAGTGTAAA pLKO_005 1044 CDS 100% 13.200 10.560 N MSH2 n/a
6 TRCN0000039669 CCTGGCAATCTCTCTCAGTTT pLKO.1 435 CDS 100% 4.950 3.465 N MSH2 n/a
7 TRCN0000332958 CCTGGCAATCTCTCTCAGTTT pLKO_005 435 CDS 100% 4.950 3.465 N MSH2 n/a
8 TRCN0000039672 GCCAGTATATGAAATTGGATA pLKO.1 952 CDS 100% 4.950 3.465 N MSH2 n/a
9 TRCN0000010383 TATAAGGCTTCTCCTGGCAAT pLKO.1 423 CDS 100% 4.050 2.835 N MSH2 n/a
10 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 6490 3UTR 100% 4.050 2.025 Y P3H4 n/a
11 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 6490 3UTR 100% 4.050 2.025 Y ORAI2 n/a
12 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 6490 3UTR 100% 4.050 2.025 Y P3H4 n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 5529 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 5529 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005264332.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01034 pDONR223 100% 93.3% 89.6% None (many diffs) n/a
2 ccsbBroad304_01034 pLX_304 0% 93.3% 89.6% V5 (many diffs) n/a
3 TRCN0000491945 TGCGAGGTAAGTAATATACCTCCG pLX_317 13% 93.3% 89.6% V5 (many diffs) n/a
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