Construct: ORF ccsbBroad304_01034
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002948.1_s304c1, BRDN0000399213
- Derived from:
- ccsbBroadEn_01034
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MSH2 (4436)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01034
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4436 | MSH2 | mutS homolog 2 | NM_000251.3 | 100% | 100% | |
| 2 | human | 4436 | MSH2 | mutS homolog 2 | XM_011532867.2 | 95.9% | 93.8% | (many diffs) |
| 3 | human | 4436 | MSH2 | mutS homolog 2 | XM_005264332.4 | 93.3% | 89.6% | (many diffs) |
| 4 | human | 4436 | MSH2 | mutS homolog 2 | NM_001258281.1 | 92.9% | 92.9% | 0_1ins198 |
| 5 | human | 4436 | MSH2 | mutS homolog 2 | XR_939685.2 | 79.4% | (many diffs) | |
| 6 | human | 4436 | MSH2 | mutS homolog 2 | XR_001738747.2 | 57.4% | (many diffs) | |
| 7 | mouse | 17685 | Msh2 | mutS homolog 2 | NM_008628.2 | 86.5% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2868
- ORF length:
- 2802
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtgcagccg aaggagacgc tgcagttgga gagcgcggcc gaggtcggct 121 tcgtgcgctt ctttcagggc atgccggaga agccgaccac cacagtgcgc cttttcgacc 181 ggggcgactt ctatacggcg cacggcgagg acgcgctgct ggccgcccgg gaggtgttca 241 agacccaggg ggtgatcaag tacatggggc cggcaggagc aaagaatctg cagagtgttg 301 tgcttagtaa aatgaatttt gaatcttttg taaaagatct tcttctggtt cgtcagtata 361 gagttgaagt ttataagaat agagctggaa ataaggcatc caaggagaat gattggtatt 421 tggcatataa ggcttctcct ggcaatctct ctcagtttga agacattctc tttggtaaca 481 atgatatgtc agcttccatt ggtgttgtgg gtgttaaaat gtccgcagtt gatggccaga 541 gacaggttgg agttgggtat gtggattcca tacagaggaa actaggactg tgtgaattcc 601 ctgataatga tcagttctcc aatcttgagg ctctcctcat ccagattgga ccaaaggaat 661 gtgttttacc cggaggagag actgctggag acatggggaa actgagacag ataattcaaa 721 gaggaggaat tctgatcaca gaaagaaaaa aagctgactt ttccacaaaa gacatttatc 781 aggacctcaa ccggttgttg aaaggcaaaa agggagagca gatgaatagt gctgtattgc 841 cagaaatgga gaatcaggtt gcagtttcat cactgtctgc ggtaatcaag tttttagaac 901 tcttatcaga tgattccaac tttggacagt ttgaactgac tacttttgac ttcagccagt 961 atatgaaatt ggatattgca gcagtcagag cccttaacct ttttcagggt tctgttgaag 1021 ataccactgg ctctcagtct ctggctgcct tgctgaataa gtgtaaaacc cctcaaggac 1081 aaagacttgt taaccagtgg attaagcagc ctctcatgga taagaacaga atagaggaga 1141 gattgaattt agtggaagct tttgtagaag atgcagaatt gaggcagact ttacaagaag 1201 atttacttcg tcgattccca gatcttaacc gacttgccaa gaagtttcaa agacaagcag 1261 caaacttaca agattgttac cgactctatc agggtataaa tcaactacct aatgttatac 1321 aggctctgga aaaacatgaa ggaaaacacc agaaattatt gttggcagtt tttgtgactc 1381 ctcttactga tcttcgttct gacttctcca agtttcagga aatgatagaa acaactttag 1441 atatggatca ggtggaaaac catgaattcc ttgtaaaacc ttcatttgat cctaatctca 1501 gtgaattaag agaaataatg aatgacttgg aaaagaagat gcagtcaaca ttaataagtg 1561 cagccagaga tcttggcttg gaccctggca aacagattaa actggattcc agtgcacagt 1621 ttggatatta ctttcgtgta acctgtaagg aagaaaaagt ccttcgtaac aataaaaact 1681 ttagtactgt agatatccag aagaatggtg ttaaatttac caacagcaaa ttgacttctt 1741 taaatgaaga gtataccaaa aataaaacag aatatgaaga agcccaggat gccattgtta 1801 aagaaattgt caatatttct tcaggctatg tagaaccaat gcagacactc aatgatgtgt 1861 tagctcagct agatgctgtt gtcagctttg ctcacgtgtc aaatggagca cctgttccat 1921 atgtacgacc agccattttg gagaaaggac aaggaagaat tatattaaaa gcatccaggc 1981 atgcttgtgt tgaagttcaa gatgaaattg catttattcc taatgacgta tactttgaaa 2041 aagataaaca gatgttccac atcattactg gccccaatat gggaggtaaa tcaacatata 2101 ttcgacaaac tggggtgata gtactcatgg cccaaattgg gtgttttgtg ccatgtgagt 2161 cagcagaagt gtccattgtg gactgcatct tagcccgagt aggggctggt gacagtcaat 2221 tgaaaggagt ctccacgttc atggctgaaa tgttggaaac tgcttctatc ctcaggtctg 2281 caaccaaaga ttcattaata atcatagatg aattgggaag aggaacttct acctacgatg 2341 gatttgggtt agcatgggct atatcagaat acattgcaac aaagattggt gctttttgca 2401 tgtttgcaac ccattttcat gaacttactg ccttggccaa tcagatacca actgttaata 2461 atctacatgt cacagcactc accactgaag agaccttaac tatgctttat caggtgaaga 2521 aaggtgtctg tgatcaaagt tttgggattc atgttgcaga gcttgctaat ttccctaagc 2581 atgtaataga gtgtgctaaa cagaaagccc tggaacttga ggagtttcag tatattggag 2641 aatcgcaagg atatgatatc atggaaccag cagcaaagaa gtgctatctg gaaagagagc 2701 aaggtgaaaa aattattcag gagttcctgt ccaaggtgaa acaaatgccc tttactgaaa 2761 tgtcagaaga aaacatcaca ataaagttaa aacagctaaa agctgaagta atagcaaaga 2821 ataatagctt tgtaaatgaa atcatttcac gaataaaagt tactacgtgc ccaactttct 2881 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2941 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta