Transcript: Human XM_005266454.4

PREDICTED: Homo sapiens SPT20 homolog, SAGA complex component (SUPT20H), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SUPT20H (55578)
Length:
2939
CDS:
217..2760

Additional Resources:

NCBI RefSeq record:
XM_005266454.4
NBCI Gene record:
SUPT20H (55578)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005266454.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000018045 GCGGATGTGTCATAGCAGAAA pLKO.1 596 CDS 100% 4.950 6.930 N SUPT20H n/a
2 TRCN0000342932 GCGGATGTGTCATAGCAGAAA pLKO_005 596 CDS 100% 4.950 6.930 N SUPT20H n/a
3 TRCN0000219055 ATAGTTCCTCAGGTAACTATT pLKO_005 1604 CDS 100% 1.320 1.056 N Supt20 n/a
4 TRCN0000081526 CAGAAGAATTACCTCCTATTT pLKO.1 533 CDS 100% 13.200 9.240 N Supt20 n/a
5 TRCN0000018044 CCACAGATGATCATTCAAATT pLKO.1 1340 CDS 100% 13.200 9.240 N SUPT20H n/a
6 TRCN0000081524 GCAGAAGAATTACCTCCTATT pLKO.1 532 CDS 100% 10.800 7.560 N Supt20 n/a
7 TRCN0000018047 GCTTGTTATGCAAGAGACTTT pLKO.1 381 CDS 100% 4.950 3.465 N SUPT20H n/a
8 TRCN0000342998 GCTTGTTATGCAAGAGACTTT pLKO_005 381 CDS 100% 4.950 3.465 N SUPT20H n/a
9 TRCN0000018043 CCATCAAGTATTCCTCGGAAA pLKO.1 1684 CDS 100% 4.050 2.835 N SUPT20H n/a
10 TRCN0000342933 CCATCAAGTATTCCTCGGAAA pLKO_005 1684 CDS 100% 4.050 2.835 N SUPT20H n/a
11 TRCN0000018046 CCCACAACTTGTAGTCCTCAA pLKO.1 2377 CDS 100% 4.050 2.835 N SUPT20H n/a
12 TRCN0000342934 CCCACAACTTGTAGTCCTCAA pLKO_005 2377 CDS 100% 4.050 2.835 N SUPT20H n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005266454.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08547 pDONR223 100% 89.2% 89% None (many diffs) n/a
2 ccsbBroad304_08547 pLX_304 0% 89.2% 89% V5 (many diffs) n/a
3 TRCN0000469326 GGACCCTGATTTTGCTTCTAACCA pLX_317 16.2% 89.2% 89% V5 (many diffs) n/a
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