Construct: ORF TRCN0000477591
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011373.1_s317c1
- Derived from:
- ccsbBroadEn_09052
- DNA Barcode:
- ATTCCCAATCATCACCCGATGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDHD1 (80821)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477591
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80821 | DDHD1 | DDHD domain containing 1 | NM_030637.3 | 99.9% | 99.8% | 2024A>G |
| 2 | human | 80821 | DDHD1 | DDHD domain containing 1 | NM_001160147.2 | 99.1% | 99% | 1011_1031del;2045A>G |
| 3 | human | 80821 | DDHD1 | DDHD domain containing 1 | NM_001160148.2 | 96.8% | 96.6% | 2024A>G;2438_2521del |
| 4 | human | 80821 | DDHD1 | DDHD domain containing 1 | XM_017021668.2 | 96.2% | 96.1% | 1012_1113del;2126A>G |
| 5 | human | 80821 | DDHD1 | DDHD domain containing 1 | XM_005268103.3 | 96.1% | 95.9% | 1011_1031del;2045A>G;2459_2542del |
| 6 | human | 80821 | DDHD1 | DDHD domain containing 1 | XM_011537189.3 | 95.4% | 95.3% | 1012_1134del;2147A>G |
| 7 | human | 80821 | DDHD1 | DDHD domain containing 1 | XM_017021669.2 | 95% | 94.9% | 1011_1012ins129;1895A>G |
| 8 | human | 80821 | DDHD1 | DDHD domain containing 1 | XM_005268102.3 | 93.3% | 93.1% | 1012_1113del;2126A>G;2540_2623del |
| 9 | human | 80821 | DDHD1 | DDHD domain containing 1 | XM_011537188.3 | 92.6% | 92.4% | 1012_1134del;2147A>G;2561_2644del |
| 10 | human | 80821 | DDHD1 | DDHD domain containing 1 | XM_005268105.3 | 92% | 91.8% | 1011_1012ins129;1895A>G;2309_2392del |
| 11 | mouse | 114874 | Ddhd1 | DDHD domain containing 1 | NM_001039106.3 | 85.6% | 88.6% | (many diffs) |
| 12 | mouse | 114874 | Ddhd1 | DDHD domain containing 1 | NM_176845.5 | 83% | 85.7% | (many diffs) |
| 13 | mouse | 114874 | Ddhd1 | DDHD domain containing 1 | NM_001042719.2 | 80% | 82.6% | (many diffs) |
| 14 | mouse | 114874 | Ddhd1 | DDHD domain containing 1 | NM_001284399.1 | 50.1% | 53.4% | (many diffs) |
| 15 | mouse | 100628600 | Mir5131 | microRNA 5131 | NR_039593.1 | 1.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2682
- ORF length:
- 2616
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttacccgggc cgcgggtccc cacggagccc cgagcataac ggccgaggcg 121 gcggcggcgg cgcctgggag ctgggctcag acgcgaggcc agcgttcggc ggcggcgtct 181 gctgcttcga gcacctgccc ggcggggacc cggacgacgg cgacgtgccc ctggccctgc 241 tgcgcgggga acccgggctg catttggcgc cgggcaccga cgaccacaac caccacctcg 301 cgctggaccc ctgcctcagt gacgagaact atgacttcag ctccgccgag tcgggctcct 361 cgctgcgcta ctacagcgag ggtgagagcg gcggcggcgg cagctccttg tcgctgcacc 421 cgccgcagca gcctccgctg gtcccgacga actcgggggg cggcggcgcg acaggagggt 481 cccccgggga aaggaaacgt acccggcttg gcggcccggc ggcccggcac cgctatgagg 541 tagtgacgga gctgggcccg gaggaggtac gctggttcta caaggaggac aagaagacct 601 ggaagccctt catcggctac gactcgctcc gcatcgagct cgccttccgg accctgctgc 661 agaccacggg tgcccggccc cagggcgggg accgggacgg cgaccatgtg tgctccccca 721 cgggcccagc ctccagttcc ggagaagatg acgatgagga ccgcgcctgc ggcttctgcc 781 agagtacgac ggggcacgag ccggagatgg tggagcttgt gaacatcgag cctgtgtgcg 841 tgcggggcgg cctctacgag gtggatgtga cccaaggaga gtgctacccg gtgtactgga 901 accaggctga taaaatacca gtaatgcgtg gacagtggtt tattgacggc acttggcagc 961 ctctagaaga ggaagaaagt aatttaattg agcaagaaca tctcaattgt tttaggggcc 1021 agcagatgca ggaaaatttc gatattgaag tgtcaaaatc catagatgga aaagatgctg 1081 ttcatagttt caagttgagt cgaaaccatg tggactggca cagtgtggat gaagtatatc 1141 tttatagtga tgcaacaaca tctaaaattg caagaacagt tacccaaaaa ctgggatttt 1201 ctaaagcatc aagtagtggt accagacttc atagaggtta tgtagaagaa gccacattag 1261 aagacaagcc atcacagact acccatattg tatttgttgt gcatggcatt gggcagaaaa 1321 tggaccaagg aagaattatc aaaaatacag ctatgatgag agaagctgca agaaaaatag 1381 aagaaaggca tttttccaac catgcaacac atgttgaatt tctgcctgtt gagtggcggt 1441 caaaacttac tcttgatgga gacactgttg attccattac tcctgacaaa gtacgaggtt 1501 taagggatat gctgaacagc agtgcaatgg acataatgta ttatactagt ccactttata 1561 gagatgaact agttaaaggc cttcagcaag agctgaatcg attgtattcc cttttctgtt 1621 ctcggaatcc agactttgaa gaaaaagggg gtaaagtctc aatagtatca cattccttgg 1681 gatgtgtaat tacttatgac ataatgactg gctggaatcc agttcggctg tatgaacagt 1741 tgctgcaaaa ggaagaagag ttgcctgatg aacgatggat gagctatgaa gaacgacatc 1801 ttcttgatga actctatata actaaacgac ggctgaagga aatagaagaa cggcttcacg 1861 gattgaaagc atcatctatg acacaaacac ctgccttaaa atttaaggtt gagaatttct 1921 tctgtatggg atccccatta gcagttttct tggcgttgcg tggcatccgc ccaggaaata 1981 ctggaagtca agaccatatt ttgcctagag agatttgtaa ccggttacta aatatttttc 2041 atcctacaga tccagtggct tatagattag aaccattaat actgaaacgc tacagcaaca 2101 tttcacctgt ccagatccac tggtacaata cttcaaatcc tttaccttat gaacatatga 2161 agccaagctt tctcaaccca gctaaagaac ctaccTCAGT TTCAGAGAAT GAAGGCATTT 2221 CAACCATACC AAGCCCTGTG ACCTCACCAG TTTTGTCCCG CCGACACTAT GGAGAATCTA 2281 TAACAAATAT AGGCAAAGCA AGCATATTAG GGGCTGCTAG CATTGGAAAG GGACTTGGAG 2341 GAATGTTGTT CTCAAGATTT GGACGTTCAT CTACAACACA GTCATCTGAA ACATCAAAAG 2401 ACTCAATGGA AGATGAGAAG AAGCCAGTTG CCTCACCTTC TGCTACCACC GTAGGGACAC 2461 AGACCCTTCC ACATAGCAGT TCTGGCTTCC TCGATTCTGC ATTGGAGTTG GATCACAGGA 2521 TTGATTTTGA ACTCAGAGAA GGCCTTGTGG AGAGCCGCTA TTGGTCAGCT GTCACGTCGC 2581 ATACTGCCTA TTGGTCATCC TTGGATGTTG CCCTTTTTCT TTTAACCTTC ATGTATAAAC 2641 ATGAGCACGA TGATGATGCA AAACCCAATT TAGATCCAAT CTGCCCAACT TTCTTGTACA 2701 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2761 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2821 AGGACGAATT CCCAATCATC ACCCGATGCT CACGCGTTAA GTCgacaatc aacctctgga 2881 ttacaaaatt tgtgaaagat t