Transcript: Human XM_005269518.4

PREDICTED: Homo sapiens PDZ domain containing 8 (PDZD8), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PDZD8 (118987)
Length:
4569
CDS:
266..3607

Additional Resources:

NCBI RefSeq record:
XM_005269518.4
NBCI Gene record:
PDZD8 (118987)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005269518.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121923 CAGGGTAAAGTTGCATCTAAA pLKO.1 3638 3UTR 100% 13.200 18.480 N PDZD8 n/a
2 TRCN0000141729 GCAGTGCTGCAAGATAACTTT pLKO.1 1508 CDS 100% 5.625 7.875 N PDZD8 n/a
3 TRCN0000140492 GATTCACTACAGAGCAGGCAT pLKO.1 3415 CDS 100% 2.640 3.696 N PDZD8 n/a
4 TRCN0000140280 GAACCCAACATGGTGTGACTA pLKO.1 2689 CDS 100% 4.950 3.960 N PDZD8 n/a
5 TRCN0000421595 CTATTAAGACGGTTGAATTAA pLKO_005 1230 CDS 100% 15.000 10.500 N Pdzd8 n/a
6 TRCN0000140152 GCTCCTAAGGGAGTACCTTTA pLKO.1 427 CDS 100% 10.800 7.560 N PDZD8 n/a
7 TRCN0000121718 CAGGCATTGAAGATATAGAAA pLKO.1 3429 CDS 100% 5.625 3.938 N PDZD8 n/a
8 TRCN0000141154 CGAATAGACAGGACACTGAAA pLKO.1 2831 CDS 100% 4.950 3.465 N PDZD8 n/a
9 TRCN0000142591 GAACCAGATGTTCTCGTTGAA pLKO.1 1973 CDS 100% 4.950 3.465 N PDZD8 n/a
10 TRCN0000141856 GATCAGGAGTTGGAACACAAT pLKO.1 3284 CDS 100% 4.950 3.465 N PDZD8 n/a
11 TRCN0000142592 GCCAAAGATGTCACTTCAGAA pLKO.1 2114 CDS 100% 4.950 3.465 N PDZD8 n/a
12 TRCN0000142554 GAGTTCAAAGATGAGGCACAA pLKO.1 1670 CDS 100% 4.050 2.835 N PDZD8 n/a
13 TRCN0000140122 GATGTGGCTTTAGGATGCCTA pLKO.1 2354 CDS 100% 2.640 1.848 N PDZD8 n/a
14 TRCN0000141058 CAGCAGCATATCAGATGACTT pLKO.1 3562 CDS 100% 4.950 2.970 N PDZD8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005269518.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04735 pDONR223 100% 96.4% 96.4% None 869_870ins123;918C>T n/a
2 ccsbBroad304_04735 pLX_304 0% 96.4% 96.4% V5 869_870ins123;918C>T n/a
3 TRCN0000492331 TCCCTGAGGAAGGAACTCCTAATC pLX_317 10.8% 96.4% 96.4% V5 869_870ins123;918C>T n/a
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