Construct: ORF TRCN0000492331
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009873.1_s317c1
- Derived from:
- ccsbBroadEn_04735
- DNA Barcode:
- TCCCTGAGGAAGGAACTCCTAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDZD8 (118987)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492331
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 118987 | PDZD8 | PDZ domain containing 8 | NM_173791.5 | 99.9% | 100% | 1041C>T |
| 2 | human | 118987 | PDZD8 | PDZ domain containing 8 | XM_005269518.4 | 96.4% | 96.4% | 869_870ins123;918C>T |
| 3 | human | 118987 | PDZD8 | PDZ domain containing 8 | XR_945601.2 | 35.2% | (many diffs) | |
| 4 | human | 118987 | PDZD8 | PDZ domain containing 8 | XM_011539265.2 | 32.2% | 32% | (many diffs) |
| 5 | human | 118987 | PDZD8 | PDZ domain containing 8 | XM_011539266.3 | 32.2% | 32% | (many diffs) |
| 6 | human | 118987 | PDZD8 | PDZ domain containing 8 | XR_945602.2 | 30.6% | (many diffs) | |
| 7 | mouse | 107368 | Pdzd8 | PDZ domain containing 8 | NM_001033222.3 | 86.6% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3528
- ORF length:
- 3462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctgctgctc atgatcctgg cgtcggccgt gctgggttcc ttcctcacgc 121 tcctcgccca gttcttcctg ctgtaccgca gacagcccga gccgccggcg gacgaggccg 181 cccgcgcggg cgagggcttc cgctacatca agccagtgcc gggcctgctc ctaagggagt 241 acctttatgg cggcggccgg gatgaggagc cctccggagc ggcccctgag ggcggcgcga 301 cccccaccgc ggcccccgag acccccgccc cgccgacgcg ggagacttgc tacttcctca 361 acgccaccat cctattcctg ttccgggagt tgcgggacac cgcgctgacc cgccgctggg 421 tcaccaagaa gatcaaggtg gagttcgagg agctgctgca gaccaagacg gccgggcgcc 481 tgctggaggg gctgagcctg cgggacgtgt tcctgggcga gacggtgccc ttcatcaaga 541 ccatccggct cgtgcggcca gtcgtgccct cggccaccgg ggagcccgat ggccctgaag 601 gggaggcgct gcccgccgcc tgccccgagg agctggcctt cgaggcggag gtggagtaca 661 acgggggctt ccacctggcc atcgacgtgg acctggtctt cggcaagtcc gcctacttgt 721 ttgtcaagct gtcccgcgtg gtgggaaggc tgcgcttggt ctttacgcgc gtgcccttca 781 cccactggtt cttctccttc gtggaagacc cgctgatcga cttcgaggtg cgctcccagt 841 ttgaagggcg gcccatgccc cagctcacct ccatcatcgt caaccagctc aagaagatca 901 tcaagcgcaa gcacacccta ccgaattaca agatcaggtt taagccgttt tttccatacc 961 agaccttgca aggatttgaa gaagatgaag agcatatcca tatacaacaa tgggcactta 1021 ctgaaggccg tcttaaagtt acgttgttag aatgtagcag gttactcatt tttggatcct 1081 atgacagaga ggcaaatgtt cattgtacac ttgagttaag cagtagtgtt tgggaagaaa 1141 aacagaggag ttctattaag acggttgaat taataaaagg aaatttacaa agtgttggac 1201 ttacacttcg tcttgtccag tcaactgatg ggtatgctgg gcacgtcatc attgaaactg 1261 tggctccaaa ctcgcctgct gcaattgcag atcttcagcg gggagatcga cttatcgcca 1321 ttggaggtgt gaaaatcaca tcaacactgc aagtgttgaa gcttatcaag caggctggtg 1381 accgagtcct ggtgtactat gaaaggcctg ttggccagag taatcaaggt gcagtgctgc 1441 aagataactt tggccagttg gaagaaaact ttttgtcaag ctcatgccaa tcgggttatg 1501 aagaggaagc tgccgggttg acagtagata ctgaaagtag agagctggat tctgaatttg 1561 aagacttggc aagtgatgtc agagcacaaa atgagttcaa agatgaggca caatcattaa 1621 gtcatagtcc caaacgtgtt ccaacaacac tttctattaa accccttgga gctatatcac 1681 cagttttaaa ccgtaaatta gctgtaggaa gtcacccact accaccgaaa attcagtcca 1741 aagatggaaa taaacctcca cccctaaaaa cttctgagat aacagaccca gcacaagtgt 1801 caaaaccaac ccaaggatct gctttcaaac cacctgtgcc accacgacca caagcgaaag 1861 ttcctttgcc ttccgccgat gctccaaatc aggcagaacc agatgttctc gttgaaaagc 1921 cagagaaggt ggtgccacct cctcttgtag ataaatctgc tgaaaagcaa gcaaaaaatg 1981 tggatgccat agacgatgca gctgcaccta agcaattttt agcaaagcaa gaagtggcca 2041 aagatgtcac ttcagaaact tcctgcccta ctaaggacag ttcggacgac cgtcaaacat 2101 gggaatcatc agaaattctt tatcgtaata agctaggaaa atggacaaga accagagcat 2161 cctgtttgtt tgacatagaa gcctgtcaca ggtacttaaa cattgcattg tggtgcaggg 2221 atcctttcaa gttgggaggt ctcatctgtt tggggcatgt tagtttaaaa cttgaagatg 2281 tggctttagg atgcctagct acatcaaaca cggaatacct ttccaaattg agactggaag 2341 ccccctcacc taaggctata gtcactagaa ccgcactacg caatctgagt atgcaaaagg 2401 gattcaatga caaattttgc tatggtgaca ttactattca cttcaaatat ttgaaagaag 2461 gagaatcaga ccaccatgta gttactaacg tagaaaaaga aaaagaaccc catttggttg 2521 aagaagtttc tgttctccct aaagaggagc aatttgttgg acagatgggt ttaacagaaa 2581 acaaacacag ttttcaggat actcagttcc agaacccaac atggtgtgac tactgtaaga 2641 aaaaagtttg gactaaagca gcttcccagt gtatgttttg tgcttatgtt tgccataaaa 2701 aatgtcaaga aaagtgtcta gctgagactt ctgtttgtgg agcaactgat aggcgaatag 2761 acaggacact gaaaaacctt aggctggaag gacaggaaac cctcttaggc ctgcctcctc 2821 gtgttgatgc tgaagctagc aagtcagtca ataaaacaac aggtttgaca aggcatatta 2881 tcaatactag ttctcgttta ttaaatttgc gtcaagtctc taaaactcgc ctttctgaac 2941 caggaaccga tctcgtagaa ccttcaccaa aacacacacc caacacgtca gacaacgaag 3001 gcagtgacac ggaggtctgt ggtccaaaca gtccttctaa acggggaaac agcacaggaa 3061 taaagttagt gagaaaagag ggtggtctgg atgacagtgt tttcattgca gttaaagaaa 3121 ttggtcgtga tctgtacagg ggcttgccta cagaggaaaG GATCCAGAAA CTAGAGTTCA 3181 TGTTGGATAA GCTACAGAAT GAAATTGATC AGGAGTTGGA ACACAATAAT TCCCTTGTTA 3241 GAGAAGAAAA AGAGACAACT GATACAAGGA AAAAATCACT TCTTTCTGCT GCCTTAGCTA 3301 AATCAGGTGA AAGGCTACAA GCTCTAACAC TTCTTATGAT TCACTACAGA GCAGGCATTG 3361 AAGATATAGA AACTTTAGAA AGTCTGTCTT TAGACCAGCA CTCCAAAAAA ATAAGCAAGT 3421 ACACAGATGA TACAGAAGAA GACCTTGATA ATGAAATAAG CCAACTAATA GACTCTCAGC 3481 CATTCAGCAG CATATCAGAT GACTTATTTG GCCCATCCGA GTCTGTGTAC CCAACTTTCT 3541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3661 GTGGAAAGGA CGATCCCTGA GGAAGGAACT CCTAATCACG CGTTAAGTCg acaatcaacc 3721 tctggattac aaaatttgtg aaagatt