Transcript: Human XM_005270691.5

PREDICTED: Homo sapiens solute carrier family 35 member A3 (SLC35A3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SLC35A3 (23443)
Length:
7273
CDS:
1880..2857

Additional Resources:

NCBI RefSeq record:
XM_005270691.5
NBCI Gene record:
SLC35A3 (23443)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005270691.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434277 GCAACCTCTTTATCGATAATA pLKO_005 2696 CDS 100% 15.000 21.000 N SLC35A3 n/a
2 TRCN0000043442 GCGTTATTCCAGAACTTTAAA pLKO.1 1951 CDS 100% 15.000 21.000 N SLC35A3 n/a
3 TRCN0000432082 AGAAGGACCTCGTTATCTATC pLKO_005 1975 CDS 100% 10.800 15.120 N SLC35A3 n/a
4 TRCN0000043441 GCTACTTATCAGGTCACGTAT pLKO.1 2210 CDS 100% 4.950 6.930 N SLC35A3 n/a
5 TRCN0000043440 CCTCAGATTCTCAGCTTGATT pLKO.1 2349 CDS 100% 5.625 3.938 N SLC35A3 n/a
6 TRCN0000043438 GCTGTTATTAAGTATGCAGAT pLKO.1 2657 CDS 100% 4.050 2.835 N SLC35A3 n/a
7 TRCN0000433417 ACTCTTTCTGTACAGATATAT pLKO_005 3232 3UTR 100% 15.000 9.000 N SLC35A3 n/a
8 TRCN0000043439 CCATCCTTGTAATAACAGCTA pLKO.1 2784 CDS 100% 2.640 1.584 N SLC35A3 n/a
9 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4672 3UTR 100% 13.200 6.600 Y LIAS n/a
10 TRCN0000166364 CACACACACACACACACACAA pLKO.1 4951 3UTR 100% 4.950 2.475 Y KAAG1 n/a
11 TRCN0000157407 GATCTCGGCTTACTGCAACTT pLKO.1 5063 3UTR 100% 4.950 2.475 Y GTF2IRD2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005270691.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11735 pDONR223 100% 65.2% 65.2% None (many diffs) n/a
2 ccsbBroad304_11735 pLX_304 0% 65.2% 65.2% V5 (many diffs) n/a
3 TRCN0000469720 GAAGGATTTCGGTCTGCAATATCG pLX_317 61.9% 65.2% 65.2% V5 (many diffs) n/a
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