Construct: ORF TRCN0000469720
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008072.1_s317c1
- Derived from:
- ccsbBroadEn_11735
- DNA Barcode:
- GAAGGATTTCGGTCTGCAATATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35A3 (23443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469720
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23443 | SLC35A3 | solute carrier family 35 me... | NM_001271684.2 | 97.5% | 97.7% | 357A>G;646_660del |
| 2 | human | 23443 | SLC35A3 | solute carrier family 35 me... | XM_011541138.3 | 81.9% | 82% | 1_126del;483A>G;772_786del |
| 3 | human | 23443 | SLC35A3 | solute carrier family 35 me... | XM_017000870.2 | 78.7% | 78.8% | (many diffs) |
| 4 | human | 23443 | SLC35A3 | solute carrier family 35 me... | XM_011541137.3 | 68.1% | 68.1% | (many diffs) |
| 5 | human | 23443 | SLC35A3 | solute carrier family 35 me... | NM_012243.3 | 65.2% | 65.2% | (many diffs) |
| 6 | human | 23443 | SLC35A3 | solute carrier family 35 me... | XM_005270691.5 | 65.2% | 65.2% | (many diffs) |
| 7 | human | 23443 | SLC35A3 | solute carrier family 35 me... | XM_011541136.2 | 65.2% | 65.2% | (many diffs) |
| 8 | human | 23443 | SLC35A3 | solute carrier family 35 me... | XM_017000871.2 | 63.7% | 63.5% | (many diffs) |
| 9 | human | 23443 | SLC35A3 | solute carrier family 35 me... | NM_001271685.2 | 57.8% | 57.7% | (many diffs) |
| 10 | human | 23443 | SLC35A3 | solute carrier family 35 me... | XM_017000869.2 | 52.8% | 52.6% | (many diffs) |
| 11 | human | 23443 | SLC35A3 | solute carrier family 35 me... | XM_011541135.3 | 46.7% | 46.5% | (many diffs) |
| 12 | mouse | 229782 | Slc35a3 | solute carrier family 35 (U... | NM_144902.3 | 58% | 61.9% | (many diffs) |
| 13 | mouse | 229782 | Slc35a3 | solute carrier family 35 (U... | XM_006501426.3 | 58% | 61.9% | (many diffs) |
| 14 | mouse | 229782 | Slc35a3 | solute carrier family 35 (U... | XM_006501425.3 | 55.9% | 59.7% | (many diffs) |
| 15 | mouse | 229782 | Slc35a3 | solute carrier family 35 (U... | XM_006501427.2 | 36.1% | 38.6% | (many diffs) |
| 16 | mouse | 229782 | Slc35a3 | solute carrier family 35 (U... | XR_001783689.1 | 9.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 711
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cgccaaccta aaatacgttt ccctgggaat tttggtcttt cagactacca 121 gtttggttct aacaatgcgt tattccagaa ctttaaaaga agaaggacct cgttatctat 181 cttctacagc agtggttgtt gctgaacttt tgaagataat ggcctgcatt ttattggtct 241 acaaagacag caaatgtagt ctaagagcac tgaatcgagt actacatgat gaaattctta 301 ataaacctat ggaaaCACTT AAACTTGCTA TTCCATCAGG GATCTATACT CTTCAGAATA 361 ATTTACTGTA TGTGGCACTA TCAAATCTAG ATGCAGCTAC TTATCAGGTC ACGTATCAGT 421 TGAAAATTCT TACAACAGCA TTATTTTCTG TGTCTATGCT TAGTAAAAAA TTGGGTGTAT 481 ACCAGTGGCT GTCCCTAGTA ATTTTGATGA CAGGAGTTGC TTTTGTACAG TGGCCCTCAG 541 ATTCTCAGCT TGATTCTAAG GAACTTTCAG CTGGTTCTCA ATTTGTAGGA CTCATGGCAG 601 TTCTCACAGC ATGTTTTTCA AGTGGCTTTG CTGGGGTTTA CTTTGAGAAA ATCTTAAAAG 661 AAACAAAACA ATCAGTGTGG ATAAGAAATA TTCAGCTTGT GTCTTTTTCC TTGGAGCCAT 721 CCTTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 841 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGAAGGATTT CGGTCTGCAA TATCGACGCG 901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt