Transcript: Mouse XM_006495471.3

PREDICTED: Mus musculus lysophospholipase 1 (Lypla1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lypla1 (18777)
Length:
2082
CDS:
175..945

Additional Resources:

NCBI RefSeq record:
XM_006495471.3
NBCI Gene record:
Lypla1 (18777)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006495471.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000076857 GCTAATCGAGATATTTCCGTT pLKO.1 730 CDS 100% 2.640 3.696 N Lypla1 n/a
2 TRCN0000324616 GCTAATCGAGATATTTCCGTT pLKO_005 730 CDS 100% 2.640 3.696 N Lypla1 n/a
3 TRCN0000076855 CCCTAATGTTTGGTTCTCTTA pLKO.1 785 CDS 100% 4.950 3.960 N Lypla1 n/a
4 TRCN0000324543 CCCTAATGTTTGGTTCTCTTA pLKO_005 785 CDS 100% 4.950 3.960 N Lypla1 n/a
5 TRCN0000076853 GCTAAGAATCAGATGGTATTA pLKO.1 1408 3UTR 100% 13.200 9.240 N Lypla1 n/a
6 TRCN0000324613 GCTAAGAATCAGATGGTATTA pLKO_005 1408 3UTR 100% 13.200 9.240 N Lypla1 n/a
7 TRCN0000076854 GCATTCCTTCTAACAGGATTA pLKO.1 674 CDS 100% 10.800 7.560 N Lypla1 n/a
8 TRCN0000324533 GCATTCCTTCTAACAGGATTA pLKO_005 674 CDS 100% 10.800 7.560 N Lypla1 n/a
9 TRCN0000052066 CAAGAAGTGAAGAATGGCATT pLKO.1 658 CDS 100% 4.050 2.835 N LYPLA1 n/a
10 TRCN0000299952 CAAGAAGTGAAGAATGGCATT pLKO_005 658 CDS 100% 4.050 2.835 N LYPLA1 n/a
11 TRCN0000076856 GCAGGAAATGATGGATGTCAA pLKO.1 888 CDS 100% 4.950 2.970 N Lypla1 n/a
12 TRCN0000324531 GCAGGAAATGATGGATGTCAA pLKO_005 888 CDS 100% 4.950 2.970 N Lypla1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006495471.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07605 pDONR223 100% 60% 61.3% None (many diffs) n/a
2 ccsbBroad304_07605 pLX_304 0% 60% 61.3% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000491531 CGAAACCGTATGGCCATTAATGCT pLX_317 48% 60% 61.3% V5 (not translated due to frame shift) (many diffs) n/a
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