Transcript: Mouse XM_006496048.3

PREDICTED: Mus musculus cyclin-dependent kinase 15 (Cdk15), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Cdk15 (271697)
Length:
1810
CDS:
112..1458

Additional Resources:

NCBI RefSeq record:
XM_006496048.3
NBCI Gene record:
Cdk15 (271697)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146647 CTCCAAGTTCAAGTAAGATG pXPR_003 AGG 298 22% 3 0.1495 Cdk15 CDK15 76485
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006496048.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360951 GAGACTCTGACATTCGTATTT pLKO_005 619 CDS 100% 13.200 18.480 N Cdk15 n/a
2 TRCN0000023260 CCGGAGTAACAGTGATTCTTT pLKO.1 324 CDS 100% 5.625 7.875 N Cdk15 n/a
3 TRCN0000023262 CTGCCTAACTACAATCCAGAA pLKO.1 1096 CDS 100% 4.050 5.670 N Cdk15 n/a
4 TRCN0000368002 ACGAAGCTTCGTGCCCTATTT pLKO_005 1504 3UTR 100% 13.200 9.240 N Cdk15 n/a
5 TRCN0000023259 GCCCAGAAGTTGAAGAGTAAA pLKO.1 298 CDS 100% 13.200 9.240 N Cdk15 n/a
6 TRCN0000368003 GCTTGAAGCATGCCAATATTG pLKO_005 569 CDS 100% 13.200 9.240 N Cdk15 n/a
7 TRCN0000023263 GCTGTATTCGAGCTAACAGAA pLKO.1 226 CDS 100% 4.950 3.465 N Cdk15 n/a
8 TRCN0000023261 GCCAATATTGTCCTCTTGCAT pLKO.1 580 CDS 100% 3.000 2.100 N Cdk15 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006496048.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12510 pDONR223 100% 70.3% 72.9% None (many diffs) n/a
2 ccsbBroad304_12510 pLX_304 0% 70.3% 72.9% V5 (many diffs) n/a
3 TRCN0000467466 AAAGGGTTGCCTAGCCCCCAAGGT pLX_317 7.9% 70.3% 72.9% V5 (many diffs) n/a
4 ccsbBroadEn_15139 pDONR223 0% 70.3% 72.9% None (many diffs) n/a
5 ccsbBroad304_15139 pLX_304 0% 70.3% 72.9% V5 (many diffs) n/a
6 TRCN0000480211 AGCAATTCTAAGCAATCTCCAACG pLX_317 35.3% 70.3% 72.9% V5 (many diffs) n/a
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