Transcript: Mouse XM_006496681.3

PREDICTED: Mus musculus kinesin-associated protein 3 (Kifap3), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kifap3 (16579)
Length:
3882
CDS:
116..2482

Additional Resources:

NCBI RefSeq record:
XM_006496681.3
NBCI Gene record:
Kifap3 (16579)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006496681.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000090943 CCCACTGATCTAAGTGTACTA pLKO.1 3630 3UTR 100% 4.950 6.930 N Kifap3 n/a
2 TRCN0000309152 CCCACTGATCTAAGTGTACTA pLKO_005 3630 3UTR 100% 4.950 6.930 N Kifap3 n/a
3 TRCN0000090946 CGGTGTCTATGGATGACTCTT pLKO.1 1884 CDS 100% 4.950 6.930 N Kifap3 n/a
4 TRCN0000315528 CGGTGTCTATGGATGACTCTT pLKO_005 1884 CDS 100% 4.950 6.930 N Kifap3 n/a
5 TRCN0000090945 CTCCTCTTAAACCTCTCGTTT pLKO.1 1262 CDS 100% 4.950 3.960 N Kifap3 n/a
6 TRCN0000090944 GCTGTTGATGAAGACCTTGAA pLKO.1 902 CDS 100% 4.950 3.465 N Kifap3 n/a
7 TRCN0000315604 GCTGTTGATGAAGACCTTGAA pLKO_005 902 CDS 100% 4.950 3.465 N Kifap3 n/a
8 TRCN0000090947 GCTCCTCTTAAACCTCTCGTT pLKO.1 1261 CDS 100% 2.640 1.848 N Kifap3 n/a
9 TRCN0000309138 GCTCCTCTTAAACCTCTCGTT pLKO_005 1261 CDS 100% 2.640 1.848 N Kifap3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006496681.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07818 pDONR223 100% 83% 90.9% None (many diffs) n/a
2 ccsbBroad304_07818 pLX_304 0% 83% 90.9% V5 (many diffs) n/a
3 TRCN0000469096 TACAAGACCATCACGAGGCATGGC pLX_317 14% 83% 90.9% V5 (many diffs) n/a
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