Transcript: Mouse XM_006497250.1

PREDICTED: Mus musculus digestive organ expansion factor homolog (zebrafish) (Diexf), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Diexf (215193)
Length:
2237
CDS:
549..1964

Additional Resources:

NCBI RefSeq record:
XM_006497250.1
NBCI Gene record:
Diexf (215193)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006497250.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000249977 CTTTGTGCGTCTCCGAAATTA pLKO_005 1562 CDS 100% 15.000 21.000 N Diexf n/a
2 TRCN0000249976 CACCAGGCCTAAGGTACTTAT pLKO_005 743 CDS 100% 13.200 10.560 N Diexf n/a
3 TRCN0000257968 TACTGCCTGCATGCCATAAAC pLKO_005 621 CDS 100% 13.200 9.240 N Diexf n/a
4 TRCN0000257943 CCGTTGAGCTGCTCATCATTG pLKO_005 1114 CDS 100% 10.800 7.560 N Diexf n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006497250.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08048 pDONR223 100% 55% 59.3% None (many diffs) n/a
2 ccsbBroad304_08048 pLX_304 0% 55% 59.3% V5 (many diffs) n/a
3 TRCN0000481121 TAGCACCTCAGTGACGGCCTCTGG pLX_317 21.7% 55% 59.3% V5 (many diffs) n/a
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