Construct: ORF TRCN0000481121
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003219.1_s317c1
- Derived from:
- ccsbBroadEn_08048
- DNA Barcode:
- TAGCACCTCAGTGACGGCCTCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UTP25 (27042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481121
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27042 | UTP25 | UTP25 small subunit process... | NM_014388.7 | 99.9% | 100% | 144A>G |
| 2 | human | 27042 | UTP25 | UTP25 small subunit process... | XM_006711275.4 | 74.7% | 73.6% | (many diffs) |
| 3 | human | 27042 | UTP25 | UTP25 small subunit process... | XM_011509418.3 | 62.3% | 62.3% | 0_1ins855 |
| 4 | mouse | 215193 | Diexf | digestive organ expansion f... | NM_145415.2 | 84.2% | 86.8% | (many diffs) |
| 5 | mouse | 215193 | Diexf | digestive organ expansion f... | XR_373895.2 | 71.9% | (many diffs) | |
| 6 | mouse | 215193 | Diexf | digestive organ expansion f... | XR_001783764.1 | 68.7% | (many diffs) | |
| 7 | mouse | 215193 | Diexf | digestive organ expansion f... | XM_006497250.1 | 55% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2334
- ORF length:
- 2268
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caaacgcggg agccggagcc agagccagct actcaacacc ctaactaaaa 121 agcagaagaa acatcttcga gatttcggcg aggagcatcc cttctatgac agggtttcca 181 gaaaggaagc aaagccacag atttgtcagc tgtcagagag ttcagattct tcagattctg 241 aaagcgactc agagagtgaa ccacaacaag tttctggcta ccacagacta cttgctacat 301 taaagaatgt ttctgaggaa gaagaggaag atgaggagga ggaagaggaa gaagacagta 361 ttgtagatga tgcagaaatg aacgatgaag atggtggtag cgatgtcagt gtggaagaag 421 agatggctgc agagtctact gaaagtccag agaatgtagc tttatctgct gaccctgagg 481 gaaaagaaga tggggaagag ccaccgggca catcacaaac atcccccgaa gagttcacag 541 atgcaaaaca cgagtcactg ttcagcctgg aaaccaattt tctggaagag gaaagtggag 601 acaactcttc tttgaaagcc tctcaagatc catttcttca acatgtgaac aaagaactga 661 aagaaaaagc aattcaggct gttgccacaa atcccaaaac tacccacgag cttaaatggc 721 ctattctggg ccagcttttc ttttcctcta agtttcagaa gttggaaaca tttaaacccc 781 caaaggatat tgacttaaag tcacttcatc tccagaagcc tctggaatcc acctggacta 841 agaccaacag ccagttccta tctggtcccc aaaaatcaag cagcccattc acccccctcc 901 agaaagaact cttcttaatt atgaattctt accgggacct gttctacccg gaaaggactg 961 ctctgaagaa cggggaagag atccgccatg tgtattgcct gcatgtgata aatcacatcc 1021 tcaaagccaa tgcccaggtg cttggcaaca atagcagacg ccgaagccag aagtttggag 1081 tgggtgatga tgatgacttc agagaccaag ggttaacaag gcccaaggta ctgatagtgg 1141 tgccattccg ggaagctgct ttgcgggtgg tgcagctctt catcagcctc ctcgagggtg 1201 acagcaagaa gaaaatcatt gtgagcaaca aaaagaggtt tcagggagaa tatggatcag 1261 atcccgagga gagaccaccc aacttgaaga ggcctgagga ttatgaagcc gtatttgtgg 1321 gcaatattga tgaccacttc aggattggag tggcaatact tcagagaagc atccgactct 1381 atgccccgtt ttactcctcg gatatcctca ttgcttcccc cctgggcttg aggaccatca 1441 ttggtggaga aggagagaag aagagagatt ttgactttct gtcttctatc gagcttctca 1501 tcattgatca agctgacatt tacctgatgc agaactggga gcatgtcctg catttgatga 1561 atcacatgaa cctactaccc ctggactcac atggggtaga cttttctcga gtgcggatgt 1621 ggagcctcaa taattggtcc aagtactatc gccagacact gctatttggg gcccttcagg 1681 atgcccagat caactcagtg ttcaacaagt actgtgtcaa catgcaaggc caggtggccg 1741 tgaggaatgt cccaatgaca ggctctatca gtcatgtcct ggtgcagctc ccacatgtct 1801 tccagaggat ggaagctgaa aacctagctt cagtgattga tgccaggTTT AACTTTTTTG 1861 TGAACAAGAT TTTGCCACAG TATCGTGATG CAGTCATGTC TCACACGCTC ATCTATATCC 1921 CCTCCTACTT TGACTTCGTG CGTCTTCGAA ATTACTTCAA GAAGGAGGAA TTGAATTTTA 1981 CCCACATCTG CGAGTACACG CAGAAGTCTG GTGTCTCCAG GGCCAGACAC TTCTTCCTTC 2041 AAGGAGAGAA ACAGTTTCTA CTTTTCACAG AGCGCTTCCA TTTCTACAAA AGGTATACAA 2101 TAAAAGGCAT CAGGAACCTG ATTTTCTATG AACTGCCGAC ATATCCACAC TTTTACAGTG 2161 AAATCTGTAA TATGCTGAGA GCCACCAACA GAGGAGAAGA GGCCACGTGG ACCTGCACTG 2221 TTCTCTACTC CAAATATGAT GCCCAGAGGT TAGCTGCCGT GGTTGGTGTG GAGCGGGCGG 2281 CACAGATGCT ACAGTCCAAC AAGAATGTCC ACCTCTTCAT TACTGGAGAA AAATGCCCAA 2341 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2401 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2461 TATCTTGTGG AAAGGACGAT AGCACCTCAG TGACGGCCTC TGGACGCGTT AAGTCgacaa 2521 tcaacctctg gattacaaaa tttgtgaaag att