Transcript: Mouse XM_006500332.2

PREDICTED: Mus musculus TELO2 interacting protein 1 (Tti1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tti1 (75425)
Length:
3870
CDS:
142..3399

Additional Resources:

NCBI RefSeq record:
XM_006500332.2
NBCI Gene record:
Tti1 (75425)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006500332.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000099279 CAACTCCAACAGCGTAGTTTA pLKO.1 2473 CDS 100% 13.200 18.480 N Tti1 n/a
2 TRCN0000332192 CAACTCCAACAGCGTAGTTTA pLKO_005 2473 CDS 100% 13.200 18.480 N Tti1 n/a
3 TRCN0000099276 CCAGGGACTTAGCACCTTAAT pLKO.1 513 CDS 100% 13.200 18.480 N Tti1 n/a
4 TRCN0000332270 CCAGGGACTTAGCACCTTAAT pLKO_005 513 CDS 100% 13.200 18.480 N Tti1 n/a
5 TRCN0000099277 GCTGCCATGATCCTTAATGAA pLKO.1 1705 CDS 100% 5.625 3.938 N Tti1 n/a
6 TRCN0000332266 GCTGCCATGATCCTTAATGAA pLKO_005 1705 CDS 100% 5.625 3.938 N Tti1 n/a
7 TRCN0000099278 GCCCTGATGATTCACAGAGAA pLKO.1 949 CDS 100% 4.950 3.465 N Tti1 n/a
8 TRCN0000332268 GCCCTGATGATTCACAGAGAA pLKO_005 949 CDS 100% 4.950 3.465 N Tti1 n/a
9 TRCN0000099275 GCTCTAAGAGTTTAACTCTTT pLKO.1 3603 3UTR 100% 0.495 0.347 N Tti1 n/a
10 TRCN0000332267 GCTCTAAGAGTTTAACTCTTT pLKO_005 3603 3UTR 100% 0.495 0.347 N Tti1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006500332.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02225 pDONR223 100% 85.2% 84.9% None (many diffs) n/a
2 ccsbBroad304_02225 pLX_304 0% 85.2% 84.9% V5 (many diffs) n/a
3 TRCN0000491682 CCAGGATCCGCGAGGCATGTCCCT pLX_317 8.6% 85.2% 84.9% V5 (many diffs) n/a
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