Transcript: Mouse XM_006500993.3

PREDICTED: Mus musculus eukaryotic translation initiation factor 4E (Eif4e), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Eif4e (13684)
Length:
1876
CDS:
10..702

Additional Resources:

NCBI RefSeq record:
XM_006500993.3
NBCI Gene record:
Eif4e (13684)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006500993.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000077475 CCGAAGATAGTGATTGGTTAT pLKO.1 619 CDS 100% 10.800 15.120 N Eif4e n/a
2 TRCN0000077477 CGATTGATCTCTAAGTTTGAT pLKO.1 229 CDS 100% 5.625 7.875 N Eif4e n/a
3 TRCN0000077474 CCTTCGATTGATCTCTAAGTT pLKO.1 225 CDS 100% 5.625 3.938 N Eif4e n/a
4 TRCN0000077476 GCAAGCAAACCTTCGATTGAT pLKO.1 216 CDS 100% 5.625 3.938 N Eif4e n/a
5 TRCN0000062577 CTGTTGTTAATGTTAGAGCTA pLKO.1 503 CDS 100% 2.640 1.848 N EIF4E n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006500993.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06150 pDONR223 100% 88.4% 92.1% None (many diffs) n/a
2 ccsbBroad304_06150 pLX_304 93.5% 88.4% 92.1% V5 (many diffs) n/a
3 TRCN0000471416 AGCTTATAGAAACCCTCGGGAACC pLX_317 54% 88.4% 92.1% V5 (many diffs) n/a
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