Transcript: Mouse XM_006501813.1

PREDICTED: Mus musculus suppressor of Ty 20 (Supt20), transcript variant X13, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Supt20 (56790)
Length:
3066
CDS:
397..2784

Additional Resources:

NCBI RefSeq record:
XM_006501813.1
NBCI Gene record:
Supt20 (56790)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006501813.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000225966 GCCAATAGGCTGCTCTATAAC pLKO_005 847 CDS 100% 13.200 18.480 N Supt20 n/a
2 TRCN0000225967 TGACGCAGAGAGGGTAGTTAA pLKO_005 1392 CDS 100% 13.200 18.480 N Supt20 n/a
3 TRCN0000081524 GCAGAAGAATTACCTCCTATT pLKO.1 541 CDS 100% 10.800 8.640 N Supt20 n/a
4 TRCN0000081526 CAGAAGAATTACCTCCTATTT pLKO.1 542 CDS 100% 13.200 9.240 N Supt20 n/a
5 TRCN0000081525 CCATTCAAACTGGTTTGTCAT pLKO.1 1359 CDS 100% 4.950 3.465 N Supt20 n/a
6 TRCN0000081527 GCAGGAGACTTTGTCGTGTTT pLKO.1 399 CDS 100% 4.950 3.465 N Supt20 n/a
7 TRCN0000219055 ATAGTTCCTCAGGTAACTATT pLKO_005 1604 CDS 100% 1.320 0.792 N Supt20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006501813.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08547 pDONR223 100% 71.2% 72.9% None (many diffs) n/a
2 ccsbBroad304_08547 pLX_304 0% 71.2% 72.9% V5 (many diffs) n/a
3 TRCN0000469326 GGACCCTGATTTTGCTTCTAACCA pLX_317 16.2% 71.2% 72.9% V5 (many diffs) n/a
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