Transcript: Mouse XM_006501998.3

PREDICTED: Mus musculus nexilin (Nexn), transcript variant X8, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nexn (68810)
Length:
2914
CDS:
507..2330

Additional Resources:

NCBI RefSeq record:
XM_006501998.3
NBCI Gene record:
Nexn (68810)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006501998.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000114148 GCATGGTATTAGATGATGATT pLKO.1 1348 CDS 100% 5.625 7.875 N Nexn n/a
2 TRCN0000114147 GCCGGAAATTACATGGTGGTT pLKO.1 2129 CDS 100% 2.640 3.696 N Nexn n/a
3 TRCN0000114146 GCATGAATAAACTGGAGATTT pLKO.1 2590 3UTR 100% 13.200 9.240 N Nexn n/a
4 TRCN0000114150 CCAAAGCTAACAGGGACAGTT pLKO.1 600 CDS 100% 4.950 3.465 N Nexn n/a
5 TRCN0000114149 CCGAGTTCTCAAAGAAGCAAA pLKO.1 953 CDS 100% 4.950 3.465 N Nexn n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006501998.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12965 pDONR223 100% 81.1% 81.8% None (many diffs) n/a
2 ccsbBroad304_12965 pLX_304 0% 81.1% 81.8% V5 (many diffs) n/a
3 TRCN0000469025 GACGATTCATCCAGGCGCGGTGTG pLX_317 27.2% 81.1% 81.8% V5 (many diffs) n/a
4 ccsbBroadEn_12966 pDONR223 100% 79.1% 79.9% None (many diffs) n/a
5 ccsbBroad304_12966 pLX_304 0% 79.1% 79.9% V5 (many diffs) n/a
6 TRCN0000480865 TCTTTGGATCACGCATAGGCGATG pLX_317 22.9% 79.1% 79.9% V5 (many diffs) n/a
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