Construct: ORF TRCN0000480865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002764.1_s317c1
- Derived from:
- ccsbBroadEn_12966
- DNA Barcode:
- TCTTTGGATCACGCATAGGCGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEXN (91624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480865
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271326.4 | 92.5% | 92.4% | 1_132del;499G>A |
| 2 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271324.5 | 90.4% | 90.3% | 1_132del;254_295del;541G>A |
| 3 | human | 91624 | NEXN | nexilin F-actin binding pro... | NM_001172309.1 | 90.1% | 89.7% | (many diffs) |
| 4 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271323.4 | 83.6% | 83.5% | 1_324del;691G>A |
| 5 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271322.4 | 81.9% | 81.8% | 1_324del;446_487del;733G>A |
| 6 | human | 91624 | NEXN | nexilin F-actin binding pro... | NM_144573.3 | 81.6% | 81.2% | (many diffs) |
| 7 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271327.4 | 80.4% | 75.2% | (many diffs) |
| 8 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271325.4 | 70.9% | 70.8% | (many diffs) |
| 9 | mouse | 68810 | Nexn | nexilin | XM_006502002.3 | 87.4% | 88.2% | (many diffs) |
| 10 | mouse | 68810 | Nexn | nexilin | XM_006502000.3 | 85.2% | 86% | (many diffs) |
| 11 | mouse | 68810 | Nexn | nexilin | XM_011240216.2 | 81% | 81.7% | (many diffs) |
| 12 | mouse | 68810 | Nexn | nexilin | NM_199465.2 | 79.1% | 79.9% | (many diffs) |
| 13 | mouse | 68810 | Nexn | nexilin | XM_006501998.3 | 79.1% | 79.9% | (many diffs) |
| 14 | mouse | 68810 | Nexn | nexilin | XM_006501996.3 | 73.1% | 73.8% | (many diffs) |
| 15 | mouse | 68810 | Nexn | nexilin | XM_006501990.3 | 71.6% | 72.3% | (many diffs) |
| 16 | mouse | 68810 | Nexn | nexilin | XM_006501991.2 | 71.6% | 72.3% | (many diffs) |
| 17 | mouse | 68810 | Nexn | nexilin | XM_006501992.3 | 71.6% | 72.3% | (many diffs) |
| 18 | mouse | 68810 | Nexn | nexilin | XM_006501993.3 | 71.6% | 72.3% | (many diffs) |
| 19 | mouse | 68810 | Nexn | nexilin | XM_011240214.2 | 71.6% | 72.3% | (many diffs) |
| 20 | mouse | 68810 | Nexn | nexilin | XM_011240215.2 | 71.4% | 71.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1731
- ORF length:
- 1662
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggnaccat ggagaaacaa agacaagagg aacaaaggaa gagaacggag gaggaacgaa 121 aacgcagaat tgagcaggat atgttagaaa agaggaaaat acagcgtgaa ttagcaaaaa 181 gggctgaaca ggaaggagat gattcactac ttataactgt ggtacctgtc aaatcatata 241 aaacatctgg aaaaatgaaa aagaattttg aggatctaga aaaagaacgt gaagagaaag 301 aaaggatcaa gtacgaggaa gataaaagaa taagatatga agaacaacga ccatctctca 361 aggaagcaaa gtgtctttca ttagttatgg atgatgaaat agaaagtgaa gcaaaaaaag 421 aatcactttc tcccagaaaa ttgaaactaa cttttgaaga actggagcga caaagacaag 481 aaaaccgaaa gaagcaagct gaagaggaag caagaaaacg tttagaagaa gagaagcgtg 541 cttttgaaga agcaaggcgg caaatggtaa atgaagatga ggaaaaccaa gacacagcaa 601 aaatttttaa agggtaccgc cctggtaaac tcaaactcag ttttgaagaa atggaaaggc 661 aaagaagaga agatgaaaaa aggaaagcag aagaagaagc cagaaggaga atagaggaag 721 aaaagaaggc gtttgctgaa gcaaggagaa atatggtagt agatgatgac tccccagaga 781 tgtataagac aatctctcaa gaatttctta caccgggaaa actggaaatt aattttgaag 841 aattattaaa acaaaaaatg gaagaagaaa aacgacgaac agaggaggaa cggaagcata 901 agctagaaat ggagaaacaa gaatttgaac aactgagaca ggaaatggga gaggaagagg 961 aagaaaatga aacctttgga ttgagcagag aatatgaaga actgatcaaa ttaaaaagga 1021 gtggctctat tcaagctaaa aacctaaaaa gcaagtttga aaaaattgga cagttgtctg 1081 aaaaagaaat acagaaaaaa atagaagaag agcgagcaag aaggagagca attgaccttg 1141 aaattaaaga gcgagaagct gaaaattttc atgaggaaga tgatgttgat gttaggcctg 1201 caagaaaaag cgaggctcca tttactcaca aagtgaatat gaaagctaga tttgaacaaa 1261 tggctaaggc aagagaagaa gaagaacaaa gaagaattga agaacaaaag ttactacgca 1321 tgcagtttga acaaagggaa attgatgcag cactaCAAAA GAAAAGAGAA GAGGAGGAGG 1381 AGGAAGAAGG TAGCATCATG AATGGCTCCA CTGCTGAAGA TGAAGAGCAA ACCAGATCAG 1441 GAGCTCCATG GTTCAAGAAG CCTCTTAAAA ACACATCAGT TGTAGACAGT GAGCCAGTCA 1501 GATTTACGGT TAAAGTAACA GGAGAACCCA AACCAGAAAT TACATGGTGG TTTGAAGGAG 1561 AAATACTGCA GGATGGAGAA GACTATCAAT ATATTGAAAG GGGAGAAACT TACTGCCTTT 1621 ACTTACCAGA AACTTTCCCA GAAGATGGAG GAGAGTATAT GTGTAAAGCA GTCAACAATA 1681 AAGGATCTGC AGCTAGTACC TGTATTCTTA CCATTGAAAC TGATGACTAC TTGCCAACTT 1741 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1801 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1861 CTTGTGGAAA GGACGATCTT TGGATCACGC ATAGGCGATG ACGCGTTAAG TCgacaatca 1921 acctctggat tacaaaattt gtgaaagatt