Construct: ORF TRCN0000469025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010802.1_s317c1
- Derived from:
- ccsbBroadEn_12965
- DNA Barcode:
- GACGATTCATCCAGGCGCGGTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEXN (91624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469025
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91624 | NEXN | nexilin F-actin binding pro... | NM_001172309.1 | 92.7% | 92.7% | 1_132del |
| 2 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271324.5 | 92.4% | 92.1% | (many diffs) |
| 3 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271326.4 | 90.1% | 89.8% | (many diffs) |
| 4 | human | 91624 | NEXN | nexilin F-actin binding pro... | NM_144573.3 | 84% | 84% | 1_324del |
| 5 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271322.4 | 83.7% | 83.4% | (many diffs) |
| 6 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271323.4 | 81.6% | 81.3% | (many diffs) |
| 7 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271327.4 | 78.7% | 72.5% | (many diffs) |
| 8 | human | 91624 | NEXN | nexilin F-actin binding pro... | XM_005271325.4 | 72.7% | 72.4% | (many diffs) |
| 9 | mouse | 68810 | Nexn | nexilin | XM_006502000.3 | 87.3% | 88.2% | (many diffs) |
| 10 | mouse | 68810 | Nexn | nexilin | XM_006502002.3 | 85.1% | 85.7% | (many diffs) |
| 11 | mouse | 68810 | Nexn | nexilin | NM_199465.2 | 81.1% | 81.8% | (many diffs) |
| 12 | mouse | 68810 | Nexn | nexilin | XM_006501998.3 | 81.1% | 81.8% | (many diffs) |
| 13 | mouse | 68810 | Nexn | nexilin | XM_011240216.2 | 78.9% | 79.5% | (many diffs) |
| 14 | mouse | 68810 | Nexn | nexilin | XM_011240215.2 | 73.6% | 74.6% | (many diffs) |
| 15 | mouse | 68810 | Nexn | nexilin | XM_006501990.3 | 73.4% | 74.1% | (many diffs) |
| 16 | mouse | 68810 | Nexn | nexilin | XM_006501991.2 | 73.4% | 74.1% | (many diffs) |
| 17 | mouse | 68810 | Nexn | nexilin | XM_006501992.3 | 73.4% | 74.1% | (many diffs) |
| 18 | mouse | 68810 | Nexn | nexilin | XM_006501993.3 | 73.4% | 74.1% | (many diffs) |
| 19 | mouse | 68810 | Nexn | nexilin | XM_011240214.2 | 73.4% | 74.1% | (many diffs) |
| 20 | mouse | 68810 | Nexn | nexilin | XM_006501996.3 | 71.5% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1770
- ORF length:
- 1701
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaaacaa agacaagagg aacaaaggaa gagaacggag gaggaacgaa 121 aacgcagaat tgagcaggat atgttagaaa agaggaaaat acagcgtgaa ttagcaaaaa 181 gggctgaaca gattgaggac ataaacaata cgggaactga atcagcatca gaggaaggag 241 atgattcact acttataact gtggtacctg tcaaatcata taaaacatct ggaaaaatga 301 aaaagaattt tgaggatcta gaaaaagaac gtgaagagaa agaaaggatc aagtacgagg 361 aagataaaag aataagatat gaagaacaac gaccatctct caaggaagca aagtgtcttt 421 cattagttat ggatgatgaa atagaaagtg aagcaaaaaa agaatcactt tctcccggaa 481 aattgaaact aacttttgaa gaactggagc gacaaagaca agaaaaccga aagaagcaag 541 ctgaagagga agcaagaaaa cgtttagaag aagagaagcg tgcttttgaa gaagcaaggc 601 ggcaaatggt aaatgaagat gaggaaaacc aagacacagc aaaaattttt aaagggtacc 661 gccctggtaa actcaaactc agttttgaag aaatggaaag gcaaagaaga gaagatgaaa 721 aaaggaaagc agaagaagaa gccagaagga gaatagagga agaaaagaag gcgtttgctg 781 aagcaaggag aaatatggta gtagatgatg actccccaga gatgtataag acaatctctc 841 aagaatttct tacaccggga aaactggaaa ttaattttga agaattatta aaacaaaaaa 901 tggaagaaga aaaacgacga acagaggagg aacggaagca taagctagaa atggagaaac 961 aagaatttga acaactgaga caggaaatgg gagaggaaga ggaagaaaat gaaacctttg 1021 gattgagcag agaatatgaa gaactgatca aattaaaaag gagtggctct attcaagcta 1081 aaaacctaaa aagcaagttt gaaaaaattg gacagttgtc tgaaaaagaa atacagaaaa 1141 aaatagaaga agagcgagca agaaggagag caattgacct tgaaattaaa gagcgagaag 1201 ctgaaaattt tcatgaggaa gatgatgttg atgttaggcc tgcaagaaaa agcgaggctc 1261 catttactca caaagtgaat atgaaagcta gatttgaaca aatggctaag gCAAGAGAAG 1321 AAGAAGAACA AAGAAGAATT GAAGAACAAA AGTTACTACG CATGCAGTTT GAACAAAGGG 1381 AAATTGATGC AGCACTACAA AAGAAAAGAG AAGAGGAGGA GGAGGAAGAA GGTAGCATCA 1441 TGAATGGCTC CACTGCTGAA GATGAAGAGC AAACCAGATC AGGAGCTCCA TGGTTCAAGA 1501 AGCCTCTTAA AAACACATCA GTTGTAGACA GTGAGCCAGT CAGATTTACG GTTAAAGTAA 1561 CAGGAGAACC CAAACCAGAA ATTACATGGT GGTTTGAAGG AGAAATACTG CAGGATGGAG 1621 AAGACTATCA ATATATTGAA AGGGGAGAAA CTTACTGCCT TTACTTACCA GAAACTTTCC 1681 CAGAAGATGG AGGAGAGTAT ATGTGTAAAG CAGTCAACAA TAAAGGATCT GCAGCTAGTA 1741 CCTGTATTCT TACCATTGAA AGTAAGAATT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1801 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1861 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGACGA 1921 TTCATCCAGG CGCGGTGTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1981 tgaaagatt