Construct: ORF TRCN0000469025

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010802.1_s317c1
Derived from:
ccsbBroadEn_12965
DNA Barcode:
GACGATTCATCCAGGCGCGGTGTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NEXN (91624)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469025
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 91624 NEXN nexilin F-actin binding pro... NM_001172309.1 92.7% 92.7% 1_132del
2 human 91624 NEXN nexilin F-actin binding pro... XM_005271324.5 92.4% 92.1% (many diffs)
3 human 91624 NEXN nexilin F-actin binding pro... XM_005271326.4 90.1% 89.8% (many diffs)
4 human 91624 NEXN nexilin F-actin binding pro... NM_144573.3 84% 84% 1_324del
5 human 91624 NEXN nexilin F-actin binding pro... XM_005271322.4 83.7% 83.4% (many diffs)
6 human 91624 NEXN nexilin F-actin binding pro... XM_005271323.4 81.6% 81.3% (many diffs)
7 human 91624 NEXN nexilin F-actin binding pro... XM_005271327.4 78.7% 72.5% (many diffs)
8 human 91624 NEXN nexilin F-actin binding pro... XM_005271325.4 72.7% 72.4% (many diffs)
9 mouse 68810 Nexn nexilin XM_006502000.3 87.3% 88.2% (many diffs)
10 mouse 68810 Nexn nexilin XM_006502002.3 85.1% 85.7% (many diffs)
11 mouse 68810 Nexn nexilin NM_199465.2 81.1% 81.8% (many diffs)
12 mouse 68810 Nexn nexilin XM_006501998.3 81.1% 81.8% (many diffs)
13 mouse 68810 Nexn nexilin XM_011240216.2 78.9% 79.5% (many diffs)
14 mouse 68810 Nexn nexilin XM_011240215.2 73.6% 74.6% (many diffs)
15 mouse 68810 Nexn nexilin XM_006501990.3 73.4% 74.1% (many diffs)
16 mouse 68810 Nexn nexilin XM_006501991.2 73.4% 74.1% (many diffs)
17 mouse 68810 Nexn nexilin XM_006501992.3 73.4% 74.1% (many diffs)
18 mouse 68810 Nexn nexilin XM_006501993.3 73.4% 74.1% (many diffs)
19 mouse 68810 Nexn nexilin XM_011240214.2 73.4% 74.1% (many diffs)
20 mouse 68810 Nexn nexilin XM_006501996.3 71.5% 72% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1770
ORF length:
1701
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagaaacaa agacaagagg aacaaaggaa gagaacggag gaggaacgaa
121aacgcagaat tgagcaggat atgttagaaa agaggaaaat acagcgtgaa ttagcaaaaa
181gggctgaaca gattgaggac ataaacaata cgggaactga atcagcatca gaggaaggag
241atgattcact acttataact gtggtacctg tcaaatcata taaaacatct ggaaaaatga
301aaaagaattt tgaggatcta gaaaaagaac gtgaagagaa agaaaggatc aagtacgagg
361aagataaaag aataagatat gaagaacaac gaccatctct caaggaagca aagtgtcttt
421cattagttat ggatgatgaa atagaaagtg aagcaaaaaa agaatcactt tctcccggaa
481aattgaaact aacttttgaa gaactggagc gacaaagaca agaaaaccga aagaagcaag
541ctgaagagga agcaagaaaa cgtttagaag aagagaagcg tgcttttgaa gaagcaaggc
601ggcaaatggt aaatgaagat gaggaaaacc aagacacagc aaaaattttt aaagggtacc
661gccctggtaa actcaaactc agttttgaag aaatggaaag gcaaagaaga gaagatgaaa
721aaaggaaagc agaagaagaa gccagaagga gaatagagga agaaaagaag gcgtttgctg
781aagcaaggag aaatatggta gtagatgatg actccccaga gatgtataag acaatctctc
841aagaatttct tacaccggga aaactggaaa ttaattttga agaattatta aaacaaaaaa
901tggaagaaga aaaacgacga acagaggagg aacggaagca taagctagaa atggagaaac
961aagaatttga acaactgaga caggaaatgg gagaggaaga ggaagaaaat gaaacctttg
1021gattgagcag agaatatgaa gaactgatca aattaaaaag gagtggctct attcaagcta
1081aaaacctaaa aagcaagttt gaaaaaattg gacagttgtc tgaaaaagaa atacagaaaa
1141aaatagaaga agagcgagca agaaggagag caattgacct tgaaattaaa gagcgagaag
1201ctgaaaattt tcatgaggaa gatgatgttg atgttaggcc tgcaagaaaa agcgaggctc
1261catttactca caaagtgaat atgaaagcta gatttgaaca aatggctaag gCAAGAGAAG
1321AAGAAGAACA AAGAAGAATT GAAGAACAAA AGTTACTACG CATGCAGTTT GAACAAAGGG
1381AAATTGATGC AGCACTACAA AAGAAAAGAG AAGAGGAGGA GGAGGAAGAA GGTAGCATCA
1441TGAATGGCTC CACTGCTGAA GATGAAGAGC AAACCAGATC AGGAGCTCCA TGGTTCAAGA
1501AGCCTCTTAA AAACACATCA GTTGTAGACA GTGAGCCAGT CAGATTTACG GTTAAAGTAA
1561CAGGAGAACC CAAACCAGAA ATTACATGGT GGTTTGAAGG AGAAATACTG CAGGATGGAG
1621AAGACTATCA ATATATTGAA AGGGGAGAAA CTTACTGCCT TTACTTACCA GAAACTTTCC
1681CAGAAGATGG AGGAGAGTAT ATGTGTAAAG CAGTCAACAA TAAAGGATCT GCAGCTAGTA
1741CCTGTATTCT TACCATTGAA AGTAAGAATT TGCCAACTTT CTTGTACAAA GTGGTTGATA
1801TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1861CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGACGA
1921TTCATCCAGG CGCGGTGTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1981tgaaagatt

Download FASTA (ORF) (Full)