Transcript: Mouse XM_006502355.3

PREDICTED: Mus musculus netrin G1 (Ntng1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ntng1 (80883)
Length:
4073
CDS:
752..2497

Additional Resources:

NCBI RefSeq record:
XM_006502355.3
NBCI Gene record:
Ntng1 (80883)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006502355.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000094518 CATGCCACTTCGTGTTTGTAT pLKO.1 1655 CDS 100% 5.625 3.938 N Ntng1 n/a
2 TRCN0000094514 CCAGACAATCTGTTAATGTAT pLKO.1 3277 3UTR 100% 5.625 3.938 N Ntng1 n/a
3 TRCN0000094516 GATGTATGTAAGAGCCTGATT pLKO.1 842 CDS 100% 4.950 3.465 N Ntng1 n/a
4 TRCN0000094515 CCTAGAGAAATCTCTCGACTA pLKO.1 1234 CDS 100% 4.050 2.835 N Ntng1 n/a
5 TRCN0000094517 CCAGCATTTCCAGTATCGGTA pLKO.1 1818 CDS 100% 2.640 1.848 N Ntng1 n/a
6 TRCN0000166364 CACACACACACACACACACAA pLKO.1 657 5UTR 100% 4.950 2.475 Y KAAG1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006502355.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02693 pDONR223 100% 68.5% 72.2% None (many diffs) n/a
2 ccsbBroad304_02693 pLX_304 0% 68.5% 72.2% V5 (many diffs) n/a
3 TRCN0000492131 AAGAGACCACGCCTCAATATTTAT pLX_317 34.4% 68.5% 72.2% V5 (many diffs) n/a
Download CSV