Transcript: Mouse XM_006507095.3

PREDICTED: Mus musculus PTPRF interacting protein, binding protein 1 (liprin beta 1) (Ppfibp1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ppfibp1 (67533)
Length:
5284
CDS:
724..3822

Additional Resources:

NCBI RefSeq record:
XM_006507095.3
NBCI Gene record:
Ppfibp1 (67533)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006507095.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000182026 CGGAATCATAGCAGGTTCTAA pLKO.1 774 CDS 100% 5.625 7.875 N Ppfibp1 n/a
2 TRCN0000197523 CCTTTGCTAAACTCATCATTA pLKO.1 4656 3UTR 100% 13.200 9.240 N Ppfibp1 n/a
3 TRCN0000002957 CGGTTAGAGCAGATGGAAGAT pLKO.1 3658 CDS 100% 4.950 3.465 N PPFIBP1 n/a
4 TRCN0000181895 GCACTCATATACCCATCAGAA pLKO.1 4559 3UTR 100% 4.950 3.465 N Ppfibp1 n/a
5 TRCN0000181654 GTAGAAACTATGGCCCAGTTA pLKO.1 3325 CDS 100% 4.950 3.465 N Ppfibp1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006507095.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07246 pDONR223 100% 84.7% 88.2% None (many diffs) n/a
2 ccsbBroad304_07246 pLX_304 0% 84.7% 88.2% V5 (many diffs) n/a
3 TRCN0000480925 TGCATACCACCTTCAATACTCTAT pLX_317 13.7% 84.7% 88.2% V5 (many diffs) n/a
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