Transcript: Mouse XM_006508333.3

PREDICTED: Mus musculus carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (Chst15), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Chst15 (77590)
Length:
5354
CDS:
1174..2859

Additional Resources:

NCBI RefSeq record:
XM_006508333.3
NBCI Gene record:
Chst15 (77590)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006508333.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000264449 GCGCGAACACTCGTGCTTATA pLKO_005 4694 3UTR 100% 13.200 18.480 N Chst15 n/a
2 TRCN0000264447 ACGCATCCAATGTCAAGTATA pLKO_005 2615 CDS 100% 13.200 10.560 N Chst15 n/a
3 TRCN0000264448 CCGAGCATTAAACTGATTATC pLKO_005 1621 CDS 100% 13.200 10.560 N Chst15 n/a
4 TRCN0000264446 TACTTCGCAAGTTCCAATAAA pLKO_005 2383 CDS 100% 15.000 10.500 N Chst15 n/a
5 TRCN0000193275 CCTGGTCTTTGGATTGATAAT pLKO.1 1416 CDS 100% 13.200 9.240 N Chst15 n/a
6 TRCN0000216005 CTACTTCGCAAGTTCCAATAA pLKO.1 2382 CDS 100% 13.200 9.240 N Chst15 n/a
7 TRCN0000264450 GCCTGGTCTTTGGATTGATAA pLKO_005 1415 CDS 100% 13.200 9.240 N Chst15 n/a
8 TRCN0000193756 CCCTTAGAATGTCCTCAGAAA pLKO.1 3876 3UTR 100% 4.950 3.465 N Chst15 n/a
9 TRCN0000154727 GAGAGGTTGTACTCAGACTAT pLKO.1 2359 CDS 100% 4.950 3.465 N CHST15 n/a
10 TRCN0000280696 GAGAGGTTGTACTCAGACTAT pLKO_005 2359 CDS 100% 4.950 3.465 N CHST15 n/a
11 TRCN0000156858 GCAGATGAACTTGCTTGCTGT pLKO.1 1323 CDS 100% 2.640 1.848 N CHST15 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006508333.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03289 pDONR223 100% 86.4% 92.5% None (many diffs) n/a
2 ccsbBroad304_03289 pLX_304 0% 86.4% 92.5% V5 (many diffs) n/a
3 TRCN0000473728 ATTGCACAACAAGCTCGTGCAGCC pLX_317 27.2% 86.4% 92.5% V5 (many diffs) n/a
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