Construct: ORF TRCN0000473728

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009852.1_s317c1
Derived from:
ccsbBroadEn_03289
DNA Barcode:
ATTGCACAACAAGCTCGTGCAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHST15 (51363)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473728
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51363 CHST15 carbohydrate sulfotransfera... NM_001270764.2 100% 100%
2 human 51363 CHST15 carbohydrate sulfotransfera... NM_015892.5 100% 100%
3 human 51363 CHST15 carbohydrate sulfotransfera... XM_005269891.3 100% 100%
4 human 51363 CHST15 carbohydrate sulfotransfera... XM_006717891.4 100% 100%
5 human 51363 CHST15 carbohydrate sulfotransfera... XM_017016319.1 100% 100%
6 human 51363 CHST15 carbohydrate sulfotransfera... XM_005269892.4 90.4% 89.8% (many diffs)
7 human 51363 CHST15 carbohydrate sulfotransfera... XM_017016320.1 90.4% 89.8% (many diffs)
8 human 51363 CHST15 carbohydrate sulfotransfera... XM_005269893.3 89.8% 88.6% (many diffs)
9 human 51363 CHST15 carbohydrate sulfotransfera... XM_017016321.1 89.8% 88.6% (many diffs)
10 human 51363 CHST15 carbohydrate sulfotransfera... XM_005269894.5 88.9% 88.9% 1496_1499delATTTinsGGC;1503_1503delCins182
11 human 51363 CHST15 carbohydrate sulfotransfera... NM_001270765.2 85.8% 78.5% (many diffs)
12 human 51363 CHST15 carbohydrate sulfotransfera... NM_014863.4 85.8% 78.5% (many diffs)
13 human 51363 CHST15 carbohydrate sulfotransfera... XM_011539857.1 69% 59.7% (many diffs)
14 mouse 77590 Chst15 carbohydrate (N-acetylgalac... NM_029935.5 86.4% 92.5% (many diffs)
15 mouse 77590 Chst15 carbohydrate (N-acetylgalac... XM_006508332.1 86.4% 92.5% (many diffs)
16 mouse 77590 Chst15 carbohydrate (N-acetylgalac... XM_006508333.3 86.4% 92.5% (many diffs)
17 mouse 77590 Chst15 carbohydrate (N-acetylgalac... XM_017312386.1 86.4% 92.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1749
ORF length:
1683
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag gcactgcatt aattgctgca tacagctgtt acccgacggc gcacacaagc
121agcaggtcaa ctgccaaggg ggcccccatc acggtcacca ggcgtgcccc acgtgcaaag
181gagaaaacaa aattctgttt cgtgtggaca gtaagcagat gaacttgctt gctgttctcg
241aagtgaggac tgaagggaac gaaaactggg gtgggttttt gcgcttcaaa aaggggaagc
301gatgtagcct cgtttttgga ctgataataa tgaccttggt aatggcttct tacatccttt
361ctggggccca ccaagagctt ctgatctcat cacctttcca ttacggaggc ttccccagca
421accccagctt gatggacagc gaaaacccaa gtgacacaaa ggagcatcac caccaatcct
481ctgtaaataa tatttcatac atgaaggact atccaagcat taaattaatt atcaacagca
541tcacaactag gattgagttc acgaccagac agctcccaga cttagaagac cttaagaagc
601aggagttgca tatgttttca gtcatcccca acaaattcct tccaaacagt aagagcccct
661gttggtacga ggagttctcg gggcagaaca ccaccgaccc ctacctcacc aactcctacg
721tgctctactc caagcgcttc cgctccacct tcgacgccct gcgcaaggcc ttctggggcc
781acctggcgca cgcgcacggg aagcacttcc gcctgcgctg cctgccgcac ttctacatca
841tagggcagcc caagtgcggg accacagacc tctatgaccg cctgcggctg caccctgagg
901tcaagttctc cgccatcaag gagccacact ggtggacccg gaagcgcttt ggaatcgtcc
961gcctaagaga tgggctgcga gaccgctatc ccgtggaaga ttatctggac ctctttgacc
1021tggccgcaca ccagatccat caaggactgc aggccagctc tgcaaaggag cagagcaaga
1081tgaatacaat cattatcggg gaggccagtg cctccacgat gtgggataat aatgcctgga
1141cgttcttcta cgacaacagc acggatggcg agccaccgtt tctgacgcag gacttcatcc
1201acgcctttca gccaaatgcc agactgattg tcatgctcag ggaccctgtg gagaggttgt
1261actcagacta tctctacttt gcaagttcga ataaaTCCGC GGACGACTTC CATGAGAAAG
1321TGACAGAAGC ACTGCAGCTG TTTGAAAATT GCATGCTTGA TTATTCACTG CGCGCCTGCG
1381TCTACAACAA CACCCTCAAC AACGCCATGC CTGTGAGGCT CCAGGTTGGG CTCTATGCTG
1441TGTACCTTCT GGACTGGCTC AGCGTTTTTG ACAAGCAACA GTTTCTCATT CTTCGCCTGG
1501AAGATCATGC ATCCAACGTC AAGTACACCA TGCACAAGGT CTTCCAGTTT CTGAACCTAG
1561GGCCCTTAAG TGAGAAGCAG GAGGCTTTGA TGACCAAGAG CCCCGCATCC AATGCACGGC
1621GTCCCGAGGA CCGGAACCTG GGGCCCATGT GGCCCATCAC ACAGAAGATT CTGCGGGATT
1681TCTACAGGCC CTTCAACGCT AGGCTGGCGC AGGTCCTCGC GGATGAGGCG TTTGCGTGGA
1741AGACGACGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1801CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1861TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATTGCA CAACAAGCTC GTGCAGCCAC
1921GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)